Bioactive grade

Recombinant human TFPI2 protein (ab167722)

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Description

  • Product name

    Recombinant human TFPI2 protein

  • Biological activity

    Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca - RPKPVE - Nval - WRK (Dnp) - NH2. The IC50 value is < 2.5 nM, as measured in 100µL reaction mixture containing 1.25 ng trypsin, 10 µM substrate, 50 mM Tris, 10 mM CaCl2, 0.15M NaCl, 0.05% Brij-35, pH 7.5.

  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    HEK 293 cells

  • Accession

    P48307

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      DAAQEPTG NNAEICLLPL DYGPCRALLL RYYYDRYTQS CRQFLYGGCE GNANNFYTWE ACDDACWRIE KVPKVCRLQV SVDDQCEGST EKYFFNLSSM TCEKFFSGGC HRNRIENRFP DEATCMGFCA PKKIPSFCYS PKDEGLCSAN VTRYYFNPRY RTCDAFTYTG CGGNDNNFVS REDCKRACAK ALK

    • Predicted molecular weight

      23 kDa including tags

    • Amino acids

      23 to 213

    • Tags

      His tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab167722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituent: 99% PBS
    Note: Normally Mannitol or Trehalose are added as protectants before lyophilization

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized protein in 50µl sterile deionized water to a final concentration of 1mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names

    • Placental protein 5
    • PP5
    • REF1
    • Retinal pigment epithelium cell factor 1
    • TFPI 2
    • TFPI-2
    • TFPI2
    • TFPI2 PEN
    • TFPI2_HUMAN
    • Tissue factor pathway inhibitor 2
    see all

  • Function

    May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin.

  • Tissue specificity

    Umbilical vein endothelial cells, liver, placenta, heart, pancreas, and maternal serum at advanced pregnancy.

  • Sequence similarities

    Contains 3 BPTI/Kunitz inhibitor domains.

  • Domain

    This inhibitor contains three inhibitory domains.

  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant human TFPI2 protein (ab167722)
    SDS-PAGE - Recombinant human TFPI2 protein (ab167722)
    SDS-PAGE of reduced ab167722 stained overnight with Coomassie Blue.

References

ab167722 has not yet been referenced specifically in any publications.

Publishing research using ab167722? Please let us know so that we can cite the reference in this datasheet.

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