Bioactive grade

Recombinant human TFPI2 protein (ab167722)

Submit a review Submit a question
SDS-PAGE - Recombinant human TFPI2 protein (ab167722)

    Key features and details

    • Expression system: HEK 293 cells
    • Purity: > 95% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/µg
    • Active: Yes
    • Tags: His tag C-Terminus
    • Suitable for: SDS-PAGE, Functional Studies

    Description

    • Product name

      Recombinant human TFPI2 protein
      See all TFPI2 proteins and peptides

    • Biological activity

      Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca - RPKPVE - Nval - WRK (Dnp) - NH2. The IC50 value is < 2.5 nM, as measured in 100µL reaction mixture containing 1.25 ng trypsin, 10 µM substrate, 50 mM Tris, 10 mM CaCl2, 0.15M NaCl, 0.05% Brij-35, pH 7.5.

    • Purity

      > 95 % SDS-PAGE.

    • Endotoxin level

      < 1.000 Eu/µg

    • Expression system

      HEK 293 cells

    • Accession

      P48307

    • Protein length

      Protein fragment

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        DAAQEPTG NNAEICLLPL DYGPCRALLL RYYYDRYTQS CRQFLYGGCE GNANNFYTWE ACDDACWRIE KVPKVCRLQV SVDDQCEGST EKYFFNLSSM TCEKFFSGGC HRNRIENRFP DEATCMGFCA PKKIPSFCYS PKDEGLCSAN VTRYYFNPRY RTCDAFTYTG CGGNDNNFVS REDCKRACAK ALK

      • Predicted molecular weight

        23 kDa including tags

      • Amino acids

        23 to 213

      • Tags

        His tag C-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab167722 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Functional Studies

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.40
      Constituent: 99% PBS

      Normally Mannitol or Trehalose are added as protectants before lyophilization

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      Reconstitute with sterile deionized water to a concentration of 400 µg/ml.

    General Info

    • Alternative names

      • Placental protein 5
      • PP5
      • REF1
      • Retinal pigment epithelium cell factor 1
      • TFPI 2
      • TFPI-2
      • TFPI2
      • TFPI2 PEN
      • TFPI2_HUMAN
      • Tissue factor pathway inhibitor 2
      see all

    • Function

      May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin.

    • Tissue specificity

      Umbilical vein endothelial cells, liver, placenta, heart, pancreas, and maternal serum at advanced pregnancy.

    • Sequence similarities

      Contains 3 BPTI/Kunitz inhibitor domains.

    • Domain

      This inhibitor contains three inhibitory domains.

    • Cellular localization

      Secreted.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant human TFPI2 protein (ab167722)
      SDS-PAGE - Recombinant human TFPI2 protein (ab167722)
      SDS-PAGE of reduced ab167722 stained overnight with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab167722? Please let us know so that we can cite the reference in this datasheet.

    ab167722 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab167722.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com