Recombinant human TGF beta 1 protein (Active) (ab116150)

Description

  • Product name

    Recombinant human TGF beta 1 protein (Active)
    See all TGF beta 1 proteins and peptides
  • Biological activity

    Method of determination: Inhibition of IL-4-induced HT-2 cell proliferation

    Acceptance criteria: ED50 ≤500 pg/mL (≥ 2.0 x 10^6 units/mg)

    The activity of Human TFG-beta is 2.2 x 10^4 RU/μg, which was calibrated against recombinant human TGF-beta 1.

  • Purity

    > 95 % SDS-PAGE.
    Protein content and purity was determined by UV spectroscopy at 280 nm; RP-HPLC calibrated against a known standard and quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    CHO cells
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CSALDTNYCF SSTEKNCCVR QLYIDFRKDL GWKWIHEPKG YHANFCLGPC PYIWSLDTQY SKVLALYNQH NPGASAAPCC VPQALEPLPI VYYVGRKPKV EQLSNMIVRS CKCS
    • Predicted molecular weight

      26 kDa
    • Amino acids

      279 to 390

Specifications

Our Abpromise guarantee covers the use of ab116150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    HPLC

    Functional Studies

    SDS-PAGE

  • Form

    Lyophilised
  • Additional notes

    Endotoxin levels determined by LAL method.

    For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at Room Temperature. Store at -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).

    Constituents: 0.1% Trifluoroacetic acid, 95% Trehalose

    Lyophilized from a sterile (0.2 micron) filtered aqueous solution.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    ab116150 should be reconstituted with sterile 10 mM HCl at a concentration of 0.1 mg/ml. When reconstituting the product, gently pipette and wash down the sides of the vial to ensure full recovery of the protein into solution. Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.

General Info

  • Alternative names

    • Cartilage-inducing factor
    • CED
    • Differentiation inhibiting factor
    • DPD1
    • LAP
    • Latency-associated peptide
    • Prepro transforming growth factor beta 1
    • TGF beta
    • TGF beta 1
    • TGF beta 1 protein
    • TGF-beta 1 protein
    • TGF-beta-1
    • TGF-beta-5
    • TGF-beta1
    • TGFB
    • Tgfb-1
    • tgfb1
    • TGFB1_HUMAN
    • TGFbeta
    • TGFbeta1
    • Transforming Growth Factor b1
    • Transforming Growth Factor beta 1
    • Transforming growth factor beta 1a
    • transforming growth factor beta-1
    • transforming growth factor, beta 1
    • Transforming Growth Factor-ß1
    see all
  • Function

    Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
  • Tissue specificity

    Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
  • Involvement in disease

    Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
  • Sequence similarities

    Belongs to the TGF-beta family.
  • Post-translational
    modifications

    Glycosylated.
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

Images

  • ab116150 Inhibited HT-2 Proliferation.

  • 1ug of ab116150 on 4-20% Tris-Glycine ge;, stained with Coomassie Blue.

    Lane 1: Non-reduced

    Lane 2: Reduced.

References

ab116150 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your enquiry.

I have reviewed with our sources the TGF beta 1 proteins that we have on our catalog. It seems the most suitable would be ab50036 TGF beta 1 protein (Active).

https://www.abcam.com/index.html?datasheet=50036 (or use the following: https://www.abcam.com/index.html?datasheet=50036).

This should be suitable for use on human cells, as it is a human protein. Unfortunately, I am sorry we do not have any information if it has been used specifically for in the in vitro induction of EMT in human cell lines, but there are many references on our website for this product which state usage of this product in a variety of applications.

https://www.abcam.com/TGF-beta-1-protein-Active-ab50036-references.html

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Answer

Thank you for contacting us.

I assume you would like to use the protein for functional studies? From the looks of it, all three of them seem to be suitable for Functional Studies, but withinAb50036the endotoxin level has been determined as well. Therefore, yes,Ab50036 looks like a good choice.

The "(active)" usually indicates that the proteinwill showe.g. it's catalyticorinhibition properties as it would ina biological system. Fromreading the datasheet I guess it has been forgotten to add this for ab50036 TGF beta 1 protein, but I will contact our datasheet department so this will be updated.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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