Recombinant Human TGT protein (ab202188)
Key features and details
- Expression system: Escherichia coli
- Purity: > 80% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: MS, SDS-PAGE
Description
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Product name
Recombinant Human TGT protein -
Purity
> 80 % SDS-PAGE.
ab202188 was purified using conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMGSEFMAGAATQASLESAPRIMRLVAECSR SRARAGELWLPHGTVATPVFMPVGTQATMKGITTEQLDALGCRICLGNTY HLGLRPGPELIQKANGLHGFMNWPHNLLTDSGGFQMVSLVSLSEVTEEGV RFRSPYDGNETLLSPEKSVQIQNALGSDIIMQLDDVVSSTVTGPRVEEAM YRSIRWLDRCIAAHQRPDKQNLFAIIQGGLDADLRATCLEEMTKRDVPGF AIGGLSGGESKSQFWRMVALSTSRLPKDKPRYLMGVGYATDLVVCVALGC DMFDCVFPTRTARFGSALVPTGNLQLRKKVFEKDFGPIDPECTCPTCQKH SRAFLHALLHSDNTAALHHLTVHNIAYQLQLMSAVRTSIVEKRFPDFVRD FMGAMYGDPTLCPTWATDALASVGITLG -
Predicted molecular weight
47 kDa including tags -
Amino acids
1 to 403 -
Tags
His tag N-Terminus -
Additional sequence information
NP_112486.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab202188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Mass Spectrometry
SDS-PAGE
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Additional notes
This product was previously labelled as QTRT1
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 30% Glycerol (glycerin, glycerine), 0.02% DTT, 69% PBS
General Info
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Alternative names
- FP3235
- Guanine insertion enzyme
- qtrt1
see all -
Function
Interacts with QTRTD1 to form an active queuine tRNA-ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). -
Pathway
tRNA modification; tRNA-queuosine biosynthesis. -
Sequence similarities
Belongs to the queuine tRNA-ribosyltransferase family. -
Cellular localization
Cytoplasm. Mitochondrion. Nucleus. Weakly associates with mitochondria, possibly via QTRTD1. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab202188 has not yet been referenced specifically in any publications.