Description

  • Product name

    Recombinant human Thioredoxin / TRX protein (Active)
    See all Thioredoxin / TRX proteins and peptides
  • Biological activity

    Specific activity is >150 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.

  • Purity

    > 95 % SDS-PAGE.
    Recombinant human Thioredoxin / TRX was overexpressed in E. coli and purified by using conventional chromatography techniques.
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MVKQIESKTA FQEALDAAGD KLVVVDFSAT WCGPCKMIKP FFHSLSEKYS NVIFLEVDVD DCQDVASECE VKCMPTFQFF KKGQKVGEFS GANKEKLEAT INELV
    • Predicted molecular weight

      12 kDa
    • Amino acids

      1 to 105
    • Additional sequence information

      (NP_003320)

Specifications

Our Abpromise guarantee covers the use of ab51064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    MW confirmed by MALDI-TOF.

    Concentration determined by BCA assay.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituent: PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • ADF
    • ATL derived factor
    • ATL-derived factor
    • DKFZp686B1993
    • MGC61975
    • SASP
    • Surface associated sulphydryl protein
    • Surface-associated sulphydryl protein
    • testicular tissue protein Li 199
    • THIO_HUMAN
    • Thioredoxin
    • thioredoxin delta 3
    • TRDX
    • Trx
    • TRX 1
    • TRX1
    • TXN
    • TXN delta 3
    • TXN protein
    • zgc:92903
    see all
  • Function

    Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
    ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
  • Sequence similarities

    Belongs to the thioredoxin family.
    Contains 1 thioredoxin domain.
  • Post-translational
    modifications

    In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.
    In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.
  • Cellular localization

    Nucleus. Cytoplasm. Secreted. Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus.
  • Information by UniProt

Images

  • 3 ug of reduced ab51064, on 15% SDS-PAGE, stained with Coomassie Blue.

  • Anti-Thioredoxin / TRX antibody (ab26320) at 1 µg/ml + Recombinant human Thioredoxin / TRX protein (Active) (ab51064) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 30 seconds

References

This product has been referenced in:

  • Wadley AJ  et al. Characterization of extracellular redox enzyme concentrations in response to exercise in humans. J Appl Physiol (1985) 127:858-866 (2019). Read more (PubMed: 31246554) »
  • Carilho Torrao RB  et al. Healthy ageing and depletion of intracellular glutathione influences T cell membrane thioredoxin-1 levels and cytokine secretion. Chem Cent J 7:150 (2013). Read more (PubMed: 24007191) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

The assay measures the difference in absorbance in a one minute reaction with insulin. If you want the unit defined, Sigma Aldritch defined it as: One unit will cause a change in A650 of 1.0 in 1 min at 25 degrees C in the insulin reduction assay. Using Sigma Aldritch's definition, ours is 7-10 units/mg. Since the amount of protein used will be proportional to the dilution factor when calculating the result, the amount of protein needed for the assay is entirely up to the researcher. We test it with 1ug, 2ug, and 4ug.

Read More

Answer

Thank you for contacting us. The ideal conditions for storing ab51064 upon delivery is aliquoting and storing at -20°C by avoiding additional freeze / thaw cycles. Theoretically storing protein at 4C for couple of weeks should not be affecting the activity of proteins. However I would suggest using the protein side by side with the new vial you have purchased. This comparison test will give you reliable tested results. We have done different temperature tests with antibodies which does not show decrease in activity at 4C for couple of weeks. Unfortunately we have not planed doing similar tests with proteins so I am sorry I cannot be more specific than what I have in second paragraph. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

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