Product nameRecombinant human Thioredoxin / TRX protein
See all Thioredoxin / TRX proteins and peptides
Protein lengthFull length protein
Amino Acid Sequence
SequenceMVKQIESKTA FQEALDAAGD KLVVVDFSAT WCGPCKMIKP FFHSLSEKYS NVIFLEVDVD DCQDVASECE VKCMPTFQFF KKGQKVGEFS GANKEKLEAT INELV
Our Abpromise guarantee covers the use of ab51064 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activitySpecific activity is 7-10 A650/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
Purity> 95 % SDS-PAGE.
Recombinant human Thioredoxin / TRX was overexpressed in E. coli and purified by using conventional chromatography techniques.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: PBS, pH 7.4
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- ATL derived factor
- ATL-derived factor
FunctionParticipates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
Sequence similaritiesBelongs to the thioredoxin family.
Contains 1 thioredoxin domain.
modificationsIn the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.
In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.
Cellular localizationNucleus. Cytoplasm. Secreted. Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus.
- Information by UniProt
This product has been referenced in:
- Carilho Torrao RB et al. Healthy ageing and depletion of intracellular glutathione influences T cell membrane thioredoxin-1 levels and cytokine secretion. Chem Cent J 7:150 (2013). Read more (PubMed: 24007191) »
- Randazzo J et al. Orally active multi-functional antioxidants are neuroprotective in a rat model of light-induced retinal damage. PLoS One 6:e21926 (2011). WB . Read more (PubMed: 21779355) »