Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human Thrombin protein (Active)
See all Thrombin proteins and peptides
The specific activity of ab269042 was >2,000 NIH units/mg in a thrombin digestion assay using recombinant GST-HER2 protein as substrate at room temperature and enzyme:substrate mass ratio of 1:120 for 1 and 3 hours, respectively.
Purity> 95 % SDS-PAGE.
Expression systemEscherichia coli
Protein lengthProtein fragment
Molecular weight information37 kDa by SDS-PAGE
Amino acids364 to 622
Additional sequence informationThrombin heavy chain
Our Abpromise guarantee covers the use of ab269042 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Constituents: 0.8% Tris HCl, 0.58% Sodium chloride, 30% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Coagulation factor II
- Coagulation factor II thrombin
- EC 18.104.22.168
FunctionThrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.
Tissue specificityExpressed by the liver and secreted in plasma.
Involvement in diseaseFactor II deficiency
Thrombophilia due to thrombin defect
Pregnancy loss, recurrent, 2
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 2 kringle domains.
Contains 1 peptidase S1 domain.
modificationsThe gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major).
Cellular localizationSecreted, extracellular space.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab269042 has not yet been referenced specifically in any publications.