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Bioactive grade

Recombinant human Thrombomodulin protein (ab98989)

Submit a review Q&A (1)References (1)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level: < 0.100 Eu/µg
  • Active: Yes
  • Suitable for: SDS-PAGE, Functional Studies

Description

  • Product name

    Recombinant human Thrombomodulin protein
    See all Thrombomodulin proteins and peptides

  • Biological activity

    Biological Activity : Measured by its ability to activate protein C induced cleavage of the chromogenic substrate, BOC-Asp-Pro Arg-AMC in the presence of thrombin. The specific activity is greater than 500 pmoles/min/ug.

  • Purity

    > 95 % SDS-PAGE.
    Purity: > 98% by SDS-PAGE gel and HPLC analyses.

  • Endotoxin level

    < 0.100 Eu/µg

  • Expression system

    HEK 293 cells

  • Accession

    P07204

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      APAEPQPGGSQCVEHDCFALYPGPATFLNASQICDGLRGHLMTVRSSVAA DVISLLLNGDGGVGRRRLWIGLQLPPGCGDPKRLGPLRGFQWVTGDNNTS YSRWARLDLNGAPLCGPLCVAVSAAEATVPSEPIWEEQQCEVKADGFLCE FHFPATCRPLAVEPGAAAAAVSITYGTPFAARGADFQALPVGSSAAVAPL GLQLMCTAPPGAVQGHWAREAPGAWDCSVENGGCEHACNAIPGAPRCQCP AGAALQADGRSCTASATQSCNDLCEHFCVPNPDQPGSYSCMCETGYRLAA DQHRCEDVDDCILEPSPCPQRCVNTQGGFECHCYPNYDLVDGECVEPVDP CFRANCEYQCQPLNQTSYLCVCAEGFAPIPHEPHRCQMFCNQTACPADCD PNTQASCECPEGYILDDGFICTDIDECENGGFCSGVCHNLPGTFECICGP DSALARHIGTDCDSGKVDGGDSGSGEPPPSPTPGSTLTPPA

    • Predicted molecular weight

      52 kDa

    • Amino acids

      19 to 509

Specifications

Our Abpromise guarantee covers the use of ab98989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilized

  • Additional notes

    For lot specific reconstitution information please contact our Scientific Support Team.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.

    Constituent: 0.164% Sodium phosphate

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    For lot specific reconstitution information please contact our Scientific Support Team.

General Info

  • Alternative names

    • AHUS 6
    • AHUS6
    • BDCA 3
    • BDCA3
    • CD 141
    • CD141
    • CD141 antigen
    • Fetomodulin
    • Thbd
    • THPH12
    • THRM
    • Thrombomodulin
    • TM
    • TRBM_HUMAN
    see all

  • Function

    Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.

  • Tissue specificity

    Endothelial cells are unique in synthesizing thrombomodulin.

  • Involvement in disease

    Defects in THBD are the cause of thrombophilia due to thrombomodulin defect (THR-THBD) [MIM:188040]. A hemostatic disorder characterized by a tendency to thrombosis.
    Defects in THBD are a cause of susceptibility to hemolytic uremic syndrome atypical type 6 (AHUS6) [MIM:612926]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.

  • Sequence similarities

    Contains 1 C-type lectin domain.
    Contains 6 EGF-like domains.

  • Post-translational
    modifications

    N-glycosylated.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.

  • Cellular localization

    Membrane.
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab98989? Please let us know so that we can cite the reference in this datasheet.

ab98989 has been referenced in 1 publication.

  • Britten MW  et al. Glycocalyx components affect platelet function, whole blood coagulation, and fibrinolysis: an in vitro study suggesting a link to trauma-induced coagulopathy. BMC Anesthesiol 21:83 (2021). PubMed: 33740916

Customer reviews and Q&As

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Question

Inquiry: Re:Thrombomodulin protein (Active) (ab98989) HI, I am interested in your product: recombinant Thrombomodulin protein (Active) (ab98989) I was wondering if you could provide me with some information. 1)Are you producing the recombinant Thrombomodulin in-house or do you use an external provider, if so, which one? (I have ordered from a different source before and would like to avoid repetition). 2)Is the recombinant Thrombomodulin expressed with its complete extracellular domain, including the Ser/Thr-rich region 3) Is it fully glycosylated, specifically with chondroiting sulfate? 4) Could you maybe provide me with an amino acid sequence? Many thanks

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Answer

Thank you for your enquiry and your interest in our products.

We produce some of our products in-house and others are sourced from suppliers whose products we believe will be of interest to our customers. This product falls into the latter category. The identity of our suppliers remains commercially sensitive information.

This product is a recombinant fragment, corresponding to amino acids 19-509 (the extracellular domain) of Human Thrombomodulin, expressed in HEK293 cells; 491 amino acids, Predicted MWt 51.5 kDa (UniProt P07204) and has only been tested in SDS-PAGE. The sequence is provided on the on-line product datasheet:

APAEPQPGGSQCVEHDCFALYPGPATFLNASQICDGLRGH LMTVRSSVAADVISLLLNGDGGVGRRRLWIGLQLPPGCGD PKRLGPLRGFQWVTGDNNTSYSRWARLDLNGAPLCGPLCV AVSAAEATVPSEPIWEEQQCEVKADGFLCEFHFPATCRPL AVEPGAAAAAVSITYGTPFAARGADFQALPVGSSAAVAPL GLQLMCTAPPGAVQGHWAREAPGAWDCSVENGGCEHACNA IPGAPRCQCPAGAALQADGRSCTASATQSCNDLCEHFCVP NPDQPGSYSCMCETGYRLAADQHRCEDVDDCILEPSPCPQ RCVNTQGGFECHCYPNYDLVDGECVEPVDPCFRANCEYQC QPLNQTSYLCVCAEGFAPIPHEPHRCQMFCNQTACPADCD PNTQASCECPEGYILDDGFICTDIDECENGGFCSGVCHNL PGTFECICGPDSALARHIGTDCDSGKVDGGDSGSGEPPPS PTPGSTLTPPA

If you need any further assistance in the future, please do not hesitate to contact me.

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