Product nameRecombinant human TIMP1 protein
See all TIMP1 proteins and peptides
Protein lengthFull length protein
SourceHEK 293 cells
Amino Acid Sequence
SequenceCVPPH PQTAFCNSDL VIRAKFVGTP EVNQTTLYQR YEIKMTKMYK GFQALGDAAD IRFVYTPAME SVCGYFHRSH NRSEEFLIAG KLQDGLLHIT TCSFVAPWNS LSLAQRRGFT KTYTVGCEEC TVFPCLSIPC KLQSGTHCLW TDQLLQGSEK GFQSRHLACL PREPGLCTWQ SLRSQIA
Molecular weight24 kDa including tags
Amino acids24 to 207
TagsHis tag C-Terminus
- Anti-TIMP1 antibody (ab126847)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-TIMP1 antibody [2A5] (ab2464)
- Anti-TIMP1 antibody - Carboxyterminal end (ab38978)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-TIMP1 antibody (ab61224)
- Anti-TIMP1 antibody (ab77847)
- Anti-6X His tag® antibody - ChIP Grade (ab9108)
Our Abpromise guarantee covers the use of ab155705 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityMeasured by its ability to inhibit human MMP2 cleavage of a fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2.The IC50 value is approximately 2.5 nM.
Endotoxin level< 1.000 Eu/µg
Purity>95% by SDS-PAGE .
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 95% PBS, 5% Trehalose
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionIt is recommended to reconstitute ab155705 in sterile PBS to a final concentration of 100 µg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage. Avoid vigorous shaking or vortexing.
- Collagenase inhibitor
- Collagenase inhibitor, Human
FunctionComplexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
Sequence similaritiesBelongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.
modificationsThe activity of TIMP1 is dependent on the presence of disulfide bonds.
- Information by UniProt
ab155705 has not yet been referenced specifically in any publications.