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  1. Link

    recombinant-human-tip49a-protein-ab98076.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
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Recombinant Human TIP49A protein (ab98076)

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SDS-PAGE - Recombinant Human TIP49A protein (ab98076)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: MS, SDS-PAGE

    Description

    • Product name

      Recombinant Human TIP49A protein
    • Purity

      > 95 % SDS-PAGE.
      ab98076 was purified using conventional chromatography techniques.
    • Expression system

      Escherichia coli
    • Accession

      Q9Y265
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        MGSSHHHHHHSSGLVPRGSHMKIEEVKSTTKTQRIASHSHVKGLGLDESG LAKQAASGLVGQENAREACGVIVELIKSKKMAGRAVLLAGPPGTGKTALA LAIAQELGSKVPFCPMVGSEVYSTEIKKTEVLMENFRRAIGLRIKETKEV YEGEVTELTPCETENPMGGYGKTISHVIIGLKTAKGTKQLKLDPSIFESL QKERVEAGDVIYIEANSGAVKRQGRCDTYATEFDLEAEEYVPLPKGDVHK KKEIIQDVTLHDLDVANARPQGGQDILSMMGQLMKPKKTEITDKLRGEIN KVVNKYIDQGIAELVPGVLFVDEVHMLDIECFTYLHRALESSIAPIVIFA SNRGNCVIRGTEDITSPHGIPLDLLDRVMIIRTMLYTPQEMKQIIKIRAQ TEGINISEEALNHLGEIGTKTTLRYSVQLLTPANLLAKINGKDSIEKEHV EEISELFYDAKSSAKILADQQDKYMK
      • Predicted molecular weight

        52 kDa including tags
      • Amino acids

        1 to 456
      • Tags

        His tag N-Terminus

    Associated products

    • Related Products

      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-TIP49A antibody [2943C1a] (ab51500)

    Specifications

    Our Abpromise guarantee covers the use of ab98076 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Mass Spectrometry

      SDS-PAGE

    • Mass spectrometry

      MALDI-TOF
    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.077% DTT, 0.316% Tris HCl, 20% Glycerol (glycerin, glycerine), 1.16% Sodium chloride

    General Info

    • Alternative names

      • 49 kDa TATA box binding protein interacting protein
      • 49 kDa TATA box-binding protein-interacting protein
      • 49 kDa TBP interacting protein
      • 49 kDa TBP-interacting protein
      • 54 kDa erythrocyte cytosolic protein
      • ECP-54
      • ECP54
      • ERYTHROCYTE CYTOSOLIC PROTEIN, 54-KD
      • INO80 complex subunit H
      • NMP 238
      • Nuclear matrix protein 238
      • PONTIN
      • Pontin 52
      • RuvB like 1
      • RuvB like AAA ATPase 1
      • RuvB-like 1
      • RUVB1_HUMAN
      • RUVBL1
      • RVB1
      • TAP54 alpha
      • TAP54-alpha
      • TIP49
      • TIP49a
      • TIP60 associated protein 54 alpha
      • TIP60-associated protein 54-alpha
      see all
    • Function

      Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex.
      May be able to bind plasminogen at cell surface and enhance plasminogen activation.
      Essential for cell proliferation.
    • Tissue specificity

      Ubiquitously expressed with high expression in heart, skeletal muscle and testis.
    • Sequence similarities

      Belongs to the ruvB family.
    • Domain

      Binding to MYC is dependent on a Myc domain essential for oncogenic activity.
    • Cellular localization

      Nucleus matrix. Nucleus > nucleoplasm. Cytoplasm. Membrane. Cytoplasm > cytoskeleton > centrosome. Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol, although it is also present in the cytoplasm and associated with the cell membranes. In prophase and prometaphase it is located at the centrosome and the branching microtubule spindles. After mitotic nuclear membrane disintigration it accumulates at the centrosome and sites of tubulin polymerization. As cells pass through metaphase and into telophase it is located close to the centrosome at the early phase of tubulin polymerization. In anaphase it accumulates at the zone of tubule interdigitation. In telophase it is found at polar tubule overlap, and it reappears at the site of chromosomal decondensation in the daughter cells.
    • Target information above from: UniProt accession Q9Y265 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human TIP49A protein (ab98076)
      SDS-PAGE - Recombinant Human TIP49A protein (ab98076)
      15% SDS-PAGE analysis of 3µg ab98076

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab98076? Please let us know so that we can cite the reference in this datasheet.

    ab98076 has not yet been referenced specifically in any publications.

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