Recombinant Human Tissue Plasminogen Activator protein (FITC) (ab92679)

Reviews (2)Q&A (4)
SDS-PAGE - Recombinant Human Tissue Plasminogen Activator protein (FITC) (ab92679)
  • SDS-PAGE - Recombinant Human Tissue Plasminogen Activator protein (FITC) (ab92679)

Key features and details

  • Expression system: CHO cells
  • Purity: > 95% SDS-PAGE
  • Suitable for: SDS-PAGE

Description

  • Product name

    Recombinant Human Tissue Plasminogen Activator protein (FITC)
    See all Tissue Plasminogen Activator proteins and peptides

  • Biological activity

    It can form 100% complex formation with PAI1.

  • Purity

    > 95 % SDS-PAGE.
    >85 percent single chain.

  • Expression system

    CHO cells

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Specifications

Our Abpromise guarantee covers the use of ab92679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid

  • Additional notes

    Protect from light.It can form 100% complex formation with PAI1.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.40
    Constituents: 9.52% HEPES, 0.58% Sodium chloride

General Info

  • Alternative names

    • Alteplase
    • DKFZp686I03148
    • Plasminogen activator tissue
    • Plasminogen activator tissue type
    • PLAT
    • Reteplase
    • t PA
    • T Plasminogen Activator
    • t-PA
    • T-plasminogen activator
    • Tissue plasminogen activator (t PA)
    • Tissue type plasminogen activator
    • Tissue-type plasminogen activator chain B
    • tPA
    • TPA_HUMAN
    • TPA1
    see all

  • Function

    Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration.

  • Tissue specificity

    Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.

  • Involvement in disease

    Note=Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism.

  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 fibronectin type-I domain.
    Contains 2 kringle domains.
    Contains 1 peptidase S1 domain.

  • Domain

    Both FN1 and one of the kringle domains are required for binding to fibrin.
    Both FN1 and EGF-like domains are important for binding to LRP1.
    The FN1 domain mediates binding to annexin A2.
    The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site.

  • Post-translational
    modifications

    The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
    Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.
    N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.
    Characterization of O-linked glycan was studied in Bowes melanoma cell line.

  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human Tissue Plasminogen Activator protein (FITC) (ab92679)
    SDS-PAGE - Recombinant Human Tissue Plasminogen Activator protein (FITC) (ab92679)
    Staining of TPA Tissue Plasminogen Activator protein by 10% SDS-PAGE (UV trans-illuminator).
    Lane 1: ab92679 (3 µg) reduced
    Lane 2: ab92679 (3 µg) non-reduced
  • SDS-PAGE - Recombinant Human Tissue Plasminogen Activator protein (FITC) (ab92679)
    SDS-PAGE - Recombinant Human Tissue Plasminogen Activator protein (FITC) (ab92679)
    Staining of TPA Tissue Plasminogen Activator protein by 10% SDS-PAGE.
    Lane 1: ab92679 (3 µg) reduced
    Lane 2: ab92679 (3 µg) non-reduced
    Lane 3: Prestained standard

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab92679? Please let us know so that we can cite the reference in this datasheet.

ab92679 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

Mouse astrocytes uptake tPA-FITC

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry
tPA-FITC ab92679 was tested in an in vitro uptake assay performed on astrocytes isolated from P2-P3 mouse pups. The astrocytes were cultured in 4 well plates coated with PDL at a density of 25.000 cells/well in DMEM, 10% horse serum and 1% antibiotica (P/S).
Before tPA application, the astrocytes were washed 2 times with pre-warmed DMEM to wash out the horse serum.
After the washes, 140 nM tPA-FITC in DMEM was applied for 15 minutes at 37 degrees. Afterwards, the cells were washed 2 times with 1xPBS and subsequently fixed with 4% PFA at room temperature.
For the ICC, after fixation, the cells were washed 2 times with 1xPBS, blocked with 1xPBS-Triton-X containing 10% NGS (goat serum) and incubated with a GFAP antibody (to visualise the astrocytes) for 1 hour at room temperature. Afterwards, the cells were washed 2 times with 1xPBS and the secondary antibody was applied together with DAPI to visualise the cell nuclei and incubated for 30 min at room temperature in the dark. After washing 3 times with 1xPBS, embedding medium was applied.
For control conditions, some cells were incubated with 140 nM tPA-FITC in DMEM for 15 minutes at 4 degrees (cells do not uptake tPA in these conditions) and some cells with 140 nM Natural Human Serum Albumin protein-FITC (ab8030) in DMEM for 15 minutes at 37 degrees.

The uploaded image shows wild type mouse astrocytes incubated with 140nM tPA for 15 minutes at 37 degrees. Scale bar is 20µm.

Abcam user community

Verified customer

Submitted May 09 2017

Other (Fluorescent Confocal Microscopy) Abreview for TPA Tissue Plasminogen Activator protein (FITC)

 Good
Abreviews
abreview image
Application
Other

Review text: Prior to using for fluoresecent confocal microscopy ab92679 (FITC-tPA) was tested for for fibrinolytic activity by measuring clot lysis using a turbidity assay. Clot lysis was observed with 20 pM ab92679.
For observation of lysis by fluorescent confocal microscopy 75 nM ab92679 was used. A signal could be detected as expected at the lysis front.
Sample: Human Recombinant protein

Primary antibody (in addition to 'TPA Tissue Plasminogen Activator protein (FITC)')
Primary antibody: None used

Secondary antibody
Secondary antibody: None used

Dr. Claire Whyte

Verified customer

Submitted Nov 27 2012

Question

Great thank you for doing this. Please find attached
fluorescent confocal image for ab92679 (confocal ab92679)
clot lysis as measured by turbidity assay for ab92679 (20 pM ab92679)
fluorescent confocal image for ab93152 (confocal ab93152).
Clot lysis as measure by turbidity assay for ab93152 (100 pM ab93152)
Best wishes,

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Answer

Thank you for sending the images.
They are now added and I also would like to confirm that the discount codes are active and ready to be used for a further purchase.
The reviews will be allocated to one of team soon and then will soon be published.

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Question

I have now submitted the abreviews as requested. I forgot to add the discount codes could you please make sure the codes will still be active. I tried to add pictures but they do not appear to have attached.
Best wishes,

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Answer

Thank you for your email Please excuse the late response, Cathrin is not in the office at the moment.
I am happy to confirm that I added the discount codes to the internal notes in the reviews and therefore the codes are no activated.
If you would like to add images to the reviews, I will be happy to do this for you if you send me the images via email.
Thank you for your valuable feedback and we wish you good luck for your research.

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Question

Thank you for your reply, I would like to accept your offer.

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Answer


I am very pleased to hear you would like to accept our offer and test ab92679 and ab93152 in Functional Studies. This code will give you: 1 free product per code before the expiration date. To redeem this offer, please submit an Abreview for Functional Studies and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Question

I am interested in your products ab92679 recombinant FITC tPA and ab93152 human FITC uPA. I have been conducting fluorescent confocal microscopy experiments to monitor fibrinolysis and would like to be able to visualise labelled tPA and uPA in this system. Could you tell me if the above products would be suitable for this.

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Answer

Thank you very much for your interest in our products.
As we haven't tested this ourself, it is hard to predict if these proteins will work in a functional study. Therefore, I can offer a discount off a future purchase if you buy ab92679 and ab93152 now, test it in functional studies and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free protein or antibody per tested product.
If you are interested in this offer, please follow these steps:
1. Reply to this e-mail to let me know that you would like to proceed and test ab92679 and ab93152 in functional study. I will then send a discount code. This code must be issued before purchasing ab92679 and ab93152 so please wait for my reply before ordering.
2. Purchase ab92679 and ab93152 either by phone, fax, or online (www.abcam.com).
3. Test it in functional study.
4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any product ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.
We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab92679 and ab93152 turn out to be unsuitable for functional study, you will still receive the discount on your next purchase after your Abreview has been submitted.
Please let me know if you have any questions about this offer and I would be happy to help you further.
The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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