Recombinant Human TLR7 protein (ab153341)

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Description

  • Product name

    Recombinant Human TLR7 protein
    See all TLR7 proteins and peptides

  • Expression system

    Wheat germ

  • Accession

    Q9NYK1

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      ARWFPKTLPCDVTLDVPKNHVIVDCTDKHLTEIPGGIPTNTTNLTLTINH IPDISPASFHRLDHLVEIDFRCNCVPIPLGSKNNMCIKRLQIKPRSFSGL

    • Predicted molecular weight

      37 kDa including tags

    • Amino acids

      27 to 126

    • Tags

      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab153341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    Western blot

  • Form

    Liquid

  • Additional notes

     

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names

    • PRO285
    • TLR 7
    • Tlr7
    • TLR7_HUMAN
    • Toll like receptor 7
    • Toll-like receptor 7
    • UNQ248
    see all

  • Function

    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.

  • Tissue specificity

    Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells.

  • Sequence similarities

    Belongs to the Toll-like receptor family.
    Contains 27 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.

  • Cellular localization

    Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human TLR7 protein (ab153341)
    SDS-PAGE - Recombinant Human TLR7 protein (ab153341)
    ab153341 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References

Publishing research using ab153341? Please let us know so that we can cite the reference in this datasheet.

ab153341 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

Is it possible for you to tell me the antibodies used to detect the proteins Human TLR7 protein fragment (ab153341) and Human TLR8 full length protein (ab153344) to generate the images displayed on your website?

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Answer

Thank you for contacting us.

Actually, no antibody was used to create these images:

As the purified, recombinant tagged fragments/proteins were used, the gels could directly be stained with Coomassie.

Coomassie stains all proteins, hence the side bands. However, as the molecular weight of the sequence and the tag is known, there is no need for a more thorough staining process.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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