Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human

Specifications

Our Abpromise guarantee covers the use of ab51945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    > 95 % SDS-PAGE.
    >98% by SDS-PAGE and HPLC analyses.
  • Form
    Lyophilised
  • Additional notes
    The reconstituted solution can be diluted into aqueous buffers or stored at –4°C for 1 week or –20°C for future use.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituent: 0.0605% Tris

    Sterile filtered and lyophilized in 5mM Tris, pH 8Endotoxin level is

  • Reconstitution
    We recommend a quick spin followed by reconstitution in 5 mM Tris, pH 8.0 to a concentration of 1 mg/ml.

General Info

  • Alternative names
    • CD120a
    • FPF
    • MGC19588
    • p55
    • p55-R
    • p60
    • TBP1
    • TBPI
    • TNF R
    • TNF R55
    • TNF-R1
    • TNF-RI
    • TNFAR
    • TNFR-I
    • TNFR1
    • TNFR55
    • TNFR60
    • TNFRI
    • TNFRSF1a
    • TNR1A_HUMAN
    • Tumor necrosis factor receptor 1
    • Tumor necrosis factor receptor superfamily, member 1A
    • Tumor necrosis factor receptor type 1
    • Tumor necrosis factor receptor type I
    • Tumor necrosis factor-binding protein 1
    see all
  • Function
    Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
  • Involvement in disease
    Familial hibernian fever
    Multiple sclerosis 5
  • Sequence similarities
    Contains 1 death domain.
    Contains 4 TNFR-Cys repeats.
  • Domain
    The domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
    Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.
  • Post-translational
    modifications
    The soluble form is produced from the membrane form by proteolytic processing.
  • Cellular localization
    Cell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
  • Information by UniProt

References

ab51945 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for confirming this information and for your help and cooperation with this case.

As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order.

Credit ID: ####


As usual if you have any further questions regarding this credit note, please contact the accounts department by email at creditcontrol@abcam.com. Please refer to the credit ID number in any correspondence with the accounting department.

I would like to wish the customer good luck with their research. The technical team is always at your service, should you require further expert advice.

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Answer

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have concerns regarding the quality of this protein.

I would like to reassure you that this protein is covered by our 6 month guarantee. I am currently reviewing this case with the originator of the protein. In order to help with our investigations, I would appreciate if you could confirm if you have tried a protein assay? Could you confirm what was the resulting protein concentration?

In the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

Thank you for your cooperation. I look forward to hearing from you with the further requested details.

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Answer

Thank you for getting back to me and for providing some further information. This is to let you know that I have just contacted and asked our Account Department to raise a credit note for you - for the cost of one vial of ab19139. For your information, the internal reference note for this credit is CN19350. You can use this credit in the near future for any of the products which are in the catalogue. I hope this helps and if I can assist further, please do not hesitate to contact me.

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Question

LOT NUMBER GR54685-1 ORDER NUMBER 988106 DESCRIPTION OF THE PROBLEM No signal or weak signal SAMPLE HEK293 cell lysate, ab51945(TNF Receptor 1 protein) PRIMARY ANTIBODY Concentration or dilution : 1:500 dilution Diluent buffer : 5% Skim-milk Incubation time : over night Incubation temperature: 4℃ Washing after primary and secondary antibodies: 1X-TBST Number of washes : 5min, three times DETECTION METHOD ECL solution (Thermo, Femto- 34095) POSITIVE AND NEGATIVE CONTROLS USED Positive control : recombinant TNFR protein-5ug(Ab51945) ANTIBODY STORAGE CONDITIONS -20℃ SAMPLE PREPARATION Lysis buffer : 1X RIPA buffer( Cell signaling #9806) Phosphatase inhibitors : sigma-P5726 Protease inhibitors : Roshe 11 873 580 001 (10XProtease inhibitor,EDTA free) Reducing agent: ß-Mercaptoethanol Boiling for ≥5 min? 10min AMOUNT OF PROTEIN LOADED protein-5ug and cell lysate -1, 2, 5, 10ug ELECTROPHORESIS/GEL CONDITIONS 12% SDS-Page Gel TRANSFER AND BLOCKING CONDITIONS Type of membrane : PVDF Protein transfer verified: YES. We confirmed protein samples on PVDF membrane by Ponceau staining as shown in below figure Blocking agent and concentration: 5% Skim-milk Blocking time:1hr Blocking temperature: room temperature SECONDARY ANTIBODY abcam 6721 Concentration or dilution 1:10000 Diluent buffer: 5% skim-milk Incubation time : 1hr Incubation temperature: room temperature Fluorochrome or enzyme conjugate: HRP conjugate Washing after primary and secondary antibodies: 1X-TBST Number of washes : 5min, three times HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 4 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? We did not alter the immune reaction processing. We just used Ab concentration variably 1:1000- 1: 500 ADDITIONAL NOTES -

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Answer

Thank you for your enquiry regardingab19139 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody. After reading through the detailed protocol you kindly forwarded to Abcam, I would like to make the following comments/suggestions: 1) Secondary antibody - detection: Does the detection system work fine? Have you used it successfully with another primary antibody? Is the HRP still active? 2) Loading: Has the customer tried loading more total protein (HEK293) onto the gel 25-30 ug per lane to see if the signal is getting stronger or not. I hope this will be useful for you. Should you still have any problem with this antibody after following these suggestions, then please do not hesitate to contact our Technical Department again.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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