Overview

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      MHHHHHHAEQVLPQALYLSNMRKAVKIRERTPEDIFKPTNGIIHHFKTMH RYTLEMFRTCQFCPQFREIIHKALIDRNIQATLESQKKLNWCREVRKLVA LKTNGDGNCLMHATSQYMWGVQDTDLVLRKALFSTLKETDTRNFKFRWQL ESLKSQEFVETGLCYDTRNWNDEWDNLIKMASTDTPMARSGLQYNSLEEI HIFVLCNILRRPIIVISDKMLRSLESGSNFAPLKVGGIYLPLHWPAQECY RYPIVLGYDSHHFVPLVTLKDSGPEIRAVPLVNRDRGRFEDLKVHFLTDP ENEMKEKLLKEYLMVIEIPVQGWDHGTTHLINAAKLDEANLPKEINLVDD YFELVQHEYKKWQ
    • Molecular weight

      43 kDa including tags
    • Amino acids

      2 to 357
    • Tags

      His tag N-Terminus
    • Additional sequence information

      NM_006290.

Specifications

Our Abpromise guarantee covers the use of ab198780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Specific Activity: 10.5 pmol/min/µg
    Assay Conditions: Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and ab198780. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, and emission = 460 nm.

  • Applications

    Functional Studies

    SDS-PAGE

  • Purity

    > 95 % SDS-PAGE.

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8
    Preservative: 1.7% Imidazole
    Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • A20
    • AISBL
    • MGC104522
    • MGC138687
    • MGC138688
    • OTU domain containing protein 7C
    • OTU domain-containing protein 7C
    • OTUD7C
    • Putative DNA binding protein A20
    • Putative DNA-binding protein A20
    • TNAP3_HUMAN
    • TNF alpha-induced protein 3
    • TNFA1P2
    • TNFAIP 3
    • TNFAIP3
    • TNFAIP3 (A20)
    • Tumor necrosis factor alpha induced protein 3
    • Tumor necrosis factor alpha-induced protein 3
    • Tumor necrosis factor induced protein 3
    • Tumor necrosis factor inducible protein A20
    • tumor necrosis factor, alpha-induced protein 3
    • Zinc finger protein A20
    see all
  • Function

    Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. In vitro able to deubiquitinate both 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system.
  • Sequence similarities

    Belongs to the peptidase C64 family.
    Contains 7 A20-type zinc fingers.
    Contains 1 OTU domain.
  • Domain

    The A20-type zinc fingers mediate the ubiquitin ligase activity.
    The OTU domain mediates the deubiquitinase activity.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

Images

  • Specific activity of ab198780.

  • 10% SDS-PAGE analysis of ab198780 with Coomassie staining.

    Lane 1:  3 µg ab198780
    Lane 2: Protein marker

References

ab198780 has not yet been referenced specifically in any publications.

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