Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 20% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human TNFAIP3 protein (Active)
See all TNFAIP3 proteins and peptides
Specific Activity: 0.8 pmol/min/µg
Assay Conditions: Reaction was performed in 50 mM Tris pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and various amount of TNFAIP3 in a volume of 50 µL. Reaction was incubated at 37°C for 30 minutes, and fluorescent signal was measured at excitation of 360 nm, and emission at 460 nm.
Purity>= 20 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
SequenceAEQVLPQALY LSNMRKAVKI RERTPEDIFK PTNGIIHHFK TMHRYTLEMF RTCQFCPQFR EIIHKALIDR NIQATLESQK KLNWCREVRK LVALKTNGDG NCLMHATSQY MWGVQDTDLV LRKALFSTLK ETDTRNFKFR WQLESLKSQE FVETGLCYDT RNWNDEWDNL IKMASTDTPM ARSGLQYNSL EEIHIFVLCN ILRRPIIVIS DKMLRSLESG SNFAPLKVGG IYLPLHWPAQ ECYRYPIVLG YDSHHFVPLV TLKDSGPEIR AVPLVNRDRG RFEDLKVHFL TDPENEMKEK LLKEYLMVIE IPVQGWDHGT THLINAAKLD EANLPKEINL VDDYFELVQH EYKKWQENSE QGRREGHAQN PMEPSVPQLS LMDVKCETPN CPFFMSVNTQ PLCHECSERR QKNQNKLPKL NSKPGPEGLP GMALGASRGE AYEPLAWNPE ESTGGPHSAP PTAPSPFLFS ETTAMKCRSP GCPFTLNVQH NGFCERCHNA RQLHASHAPD HTRHLDPGKC QACLQDVTRT FNGICSTCFK RTTAEASSSL STSLPPSCHQ RSKSDPSRLV RSPSPHSCHR AGNDAPAGCL SQAARTPGDR TGTSKCRKAG CVYFGTPENK GFCTLCFIEY RENKHFAAAS GKVSPTASRF QNTIPCLGRE CGTLGSTMFE GYCQKCFIEA QNQRFHEAKR TEEQLRSSQR RDVPRTTQST SRPKCARASC KNILACRSEE LCMECQHPNQ RMGPGAHRGE PAPEDPPKQR CRAPACDHFG NAKCNGYCNE CFQFKQMYG
Predicted molecular weight91 kDa including tags
Amino acids2 to 790
TagsDDDDK tag N-Terminus
Our Abpromise guarantee covers the use of ab271357 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.05% Tween, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
80 µg/ml DDDDK peptide
This product is an active protein and may elicit a biological response in vivo, handle with caution.
FunctionUbiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. In vitro able to deubiquitinate both 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system.
Sequence similaritiesBelongs to the peptidase C64 family.
Contains 7 A20-type zinc fingers.
Contains 1 OTU domain.
DomainThe A20-type zinc fingers mediate the ubiquitin ligase activity.
The OTU domain mediates the deubiquitinase activity.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271357 has not yet been referenced specifically in any publications.