Bioactive grade

Recombinant human TNFRSF14/HVEM protein (Active) (ab219714)

Description

  • Product name

    Recombinant human TNFRSF14/HVEM protein (Active)
    See all TNFRSF14/HVEM proteins and peptides
  • Biological activity

    Measured by its binding ability in a functional ELISA.

    Immobilized ab219714 at 5 μg/mL (100 μ L/well) can bind Human BTLA, Fc Tag with a linear range of 0.08-0.3 μ g/mL. 

    Immobilized ab219714 at 5 μg/mL (100 μL/well) can bind Human BTLA (31-150), Fc Tag with a linear range of 0.08-1.25 μg/mL.

  • Purity

    > 90 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    HEK 293 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      LPSCKEDEYPVGSECCPKCSPGYRVKEACGELTGTVCEPCPPGTYIAHLN GLSKCLQCQMCDPAMGLRASRNCSRTENAVCGCSPGHFCIVQDGDHCAAC RAYATSSPGQRVQKGGTESQDTLCQNCPPGTFSPNGTLEECQHQTKCSWL VTKAGAGTSSSHWV
    • Predicted molecular weight

      19 kDa including tags
    • Amino acids

      39 to 202
    • Tags

      His tag C-Terminus
    • Additional sequence information

      (Accession # NP_003811).

Specifications

Our Abpromise guarantee covers the use of ab219714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Lyophilised
  • Additional notes

     This product was previously labelled as TNFRSF14

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 1mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names

    • HVEML
    • ATAR
    • CD270
    • CD40 like protein precursor
    • Herpes virus entry mediator A
    • Herpesvirus entry mediator
    • Herpesvirus entry mediator A
    • Herpesvirus entry mediator ligand
    • HveA
    • HVEM
    • HVEM L
    • LIGHT
    • LIGHTR
    • TNFRSF14
    • TNFSF 14
    • TNR14_HUMAN
    • TR2
    • Tumor necrosis factor receptor like gene2
    • Tumor necrosis factor receptor superfamily member 14
    • Tumor necrosis factor receptor superfamily member 14 precursor
    • Tumor necrosis factor receptor-like 2
    see all
  • Function

    Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells.
  • Tissue specificity

    Widely expressed, with the highest expression in lung, spleen and thymus.
  • Sequence similarities

    Contains 3 TNFR-Cys repeats.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Membrane.
  • Information by UniProt

Images

  • Immobilized Human HVEM, His Tag at 0.5 µg/mL (100 µL/well) can bind Human BTLA, Fc Tag with a linear range of 0.078-2.5 µg/mL.
  • Immobilized Human LIGHT, Mouse IgG2a Fc Tag, low endotoxin at 2 µg/mL (100 µL/well) can bind Human HVEM, His Tag with a linear range of 0.04-0.6 µg/mL.
  • SDS-PAGE analysis of reduced ab219714 stained overnight with Coomassie Blue.

  • Immobilized ab219714 at 5 μg/mL (100 μL/well) can bind Human BTLA (31-150), Fc Tag with a linear range of 0.08 – 1.25 μg/mL.

  • Immobilized Human ab219714 at 5 μg/mL (100 μL/well) can bind Human BTLA, Fc Tag with a linear range of 0.08 – 0.3 μg/mL.

References

ab219714 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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