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    recombinant-human-tnfsf18gitrl-protein-ab50093.pdf

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Immunology Innate Immunity Cytokines TNF Superfamily
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Recombinant human TNFSF18/GITRL protein (ab50093)

  • Datasheet
  • SDS
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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Active: Yes
  • Suitable for: SDS-PAGE, Functional Studies

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ELISA
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Human TNFSF18 / GITRL ELISA Kit (ab113323)

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Description

  • Product name

    Recombinant human TNFSF18/GITRL protein
    See all TNFSF18/GITRL proteins and peptides
  • Biological activity

    Biological activity: Use at a concentration of 1 - 10 ng/ml to stimulate IL8 production by human PBMC. Results may vary with PBMC donors.

  • Purity

    > 95 % SDS-PAGE.
    Greater than 98% by SDS-PAGE and HPLC analyses.
  • Expression system

    Escherichia coli
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      METAKEPCMAKFGPLPSKWQMASSEPPCVNKVSDWKLEILQNGLYLIYGQ VAPNANYNDVAPFEVRLYKNKDMIQTLTNKSKIQNVGGTYELHVGDTIDL IFNSEHQVLKNNTYWGIILLANPQFI
    • Amino acids

      52 to 176
    • Additional sequence information

      Contains 127 amino acid residues corresponding to the extracellular domain of TNFS18 / GITRL.

Associated products

    Specifications

    Our Abpromise guarantee covers the use of ab50093 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Functional Studies

    • Form

      Lyophilized
    • Additional notes

      Protein previously known as TNFSF18.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4 degC for 1 week or -20 degC for future use.

    General Info

    • Alternative names

      • Activation inducible TNF related ligand
      • Activation-inducible TNF-related ligand
      • AITR ligand
      • AITRL
      • GITR ligand
      • GITRL
      • Glucocorticoid induced TNF related ligand
      • Glucocorticoid induced TNFR related protein ligand
      • Glucocorticoid-induced TNF-related ligand
      • hGITRL
      • MGC138237
      • TL6
      • TNF18_HUMAN
      • TNFSF18
      • TNLG2A
      • Tumor necrosis factor (ligand) superfamily member 18
      • Tumor necrosis factor ligand 2A
      • Tumor necrosis factor ligand superfamily member 18
      see all
    • Function

      Cytokine that binds to TNFRSF18/AITR/GITR. Important for interactions between activated T-lymphocytes and endothelial cells and may modulate T-lymphocyte survival in peripheral tissues.
    • Tissue specificity

      Expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells.
    • Sequence similarities

      Belongs to the tumor necrosis factor family.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession Q9UNG2 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab50093? Please let us know so that we can cite the reference in this datasheet.

    ab50093 has not yet been referenced specifically in any publications.

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