Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human

Specifications

Our Abpromise guarantee covers the use of ab55764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Biological Activity: Determined by its ability to induce apoptotic cell death in TRAIL-sensitive U343MG cells. The expected ED50 for this effect is 1.0-3.0 ng/ml.
  • Applications

    SDS-PAGE

    Functional Studies

  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.

    Preservative: None

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted in water or other buffer solutions. Reconstituted protein should be stored in working aliquots at -20°C.

General Info

  • Alternative names
    • Apo 2 ligand
    • APO 2L
    • Apo-2 ligand
    • Apo-2L
    • APO2L
    • CD253
    • CD253 antigen
    • Chemokine tumor necrosis factor ligand superfamily member 10
    • Protein TRAIL
    • TL2
    • TNF Related Apoptosis Inducing Ligand
    • TNF related apoptosis inducing ligand TRAIL
    • TNF-related apoptosis-inducing ligand
    • TNF10_HUMAN
    • TNFSF10
    • TRAIL
    • Tumor necrosis factor (ligand) family member 10
    • Tumor Necrosis Factor (ligand) Superfamily Member 10
    • Tumor necrosis factor apoptosis inducing ligand splice variant delta
    • Tumor necrosis factor ligand superfamily member 10
    see all
  • Function
    Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
  • Tissue specificity
    Widespread; most predominant in spleen, lung and prostate.
  • Sequence similarities
    Belongs to the tumor necrosis factor family.
  • Cellular localization
    Membrane.
  • Information by UniProt

References

ab55764 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your enquiry.

I am sorry to confirm that non of these peptides have been tested in Western blot. All tested applications covered by the 6 month guarantee are listed on the individual product datasehets.

Please note that ab7993 JNK1 peptide is very small, KNGVIRGQPSPLAQVQQ, as stated on the datsheet. This will have a very low molecular weight and is regrettably therefore likely to run off the gel.

I can suggest JNK1 protein (Tagged) (ab43627) or JNK1 protein (Active) (ab60304)
would be more appropriate as a control in western blotting, if your antibody detects in mouse. Please note these are mouse protein rather than human protein, but they have been tested in WB.

https://www.abcam.com/index.html?datasheet=43627 (or use the following: https://www.abcam.com/index.html?datasheet=43627).

https://www.abcam.com/index.html?datasheet=60304 (or use the following: https://www.abcam.com/index.html?datasheet=60304

ab71596 ROCK2 peptide is also a very small peptide (NQSIRRPSRQLAPNKPS) which is also likely to run off the gel.

I can suggest ROCK2 protein (Active) (ab125828) would be more suitable. This is human protein and tested in WB
https://www.abcam.com/index.html?datasheet=125828 (or use the following: https://www.abcam.com/index.html?datasheet=125828).

ab55764 TRAIL protein is large enough to be detectable on a western blot, and you would need to add loading buffer to reduce and denature.This has been tested in SDS page. Please note this is not full length protein (from datasheet: Recombinant human soluble TRAIL is a 168 amino acid polypeptide (19.6 kDa), consisting of the extracellular domain of full length TRAIL), soyou will need to use the right antibody to ensure it has an epitope within the extracellular domain, which this peptide is taken from.

I am sorry the ones you purchased may not suitable for your applications on this occasion, but I hope the others will be more helpful. I can recommend to review the datasheets for further information. If you have any further questions, please do not hesitate to contact me.

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Question
Answer

Thank you for contacting us.
I have attached the data for the Functional Studies done with ab2219. We will add these to the datasheet as well very soon.
Also, I wanted to send you the information about our 3 for 2 promotion for all proteins that is going on until the end of the month.
https://www.abcam.com/index.html?pageconfig=resource&rid=15079
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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