Description

  • Product name

    Recombinant human TRAP1 protein
  • Biological activity

    SPECIFIC ACTIVITY:

    Kd = 1.95 nM. Assay conditions: 20 mM HEPES, pH 7.5, 50 mM KCl, 5 mM MgCl2, 20 mM sodium molybdate, 2 mM DTT, 0.01% NP40, 100 μg/ml BSA, 50 nM TRAP1, geldanamycin-FITC (titration range 0.016-16 nM) incubated at 4oC for 24 hrs. Fluorescence polarization measured at RT (Ex/Em= 485/520 nm)

  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      77 kDa including tags
    • Tags

      DDDDK tag C-Terminus
    • Additional sequence information

      Immunogen length = 702 amino acid residues

Specifications

Our Abpromise guarantee covers the use of ab123775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Liquid
  • Additional notes

    Kd = 1.95 nM. Assay conditions: 20 mM HEPES, pH 7.5, 50 mM KCl, 5 mM MgCl2, 20 mM sodium molybdate, 2 mM DTT, 0.01% NP40, 100 µg/ml BSA, 50 nM TRAP1, geldanamycin-FITC (titration range 0.016-16 nM) incubated at 4oC for 24 hrs. Fluorescence polarization measured at RT (Ex/Em= 485/520 nm).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.40
    Constituents: 1.06% Potassium phosphate, 0.008% DTT, 0.015% EDTA, 2.5% Glycerol, 0.29% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Heat shock protein 75 kDa
    • Heat shock protein 75 kDa, mitochondrial
    • HSP 75
    • HSP75
    • HSP90L
    • mitochondrial
    • TNF receptor associated protein 1
    • TNFR-associated protein 1
    • TRAP-1
    • Trap1
    • TRAP1_HUMAN
    • Tumor necrosis factor type 1 receptor-associated protein
    see all
  • Function

    Chaperone that expresses an ATPase activity.
  • Tissue specificity

    Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta.
  • Sequence similarities

    Belongs to the heat shock protein 90 family.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt

Images

  • 10% SDS-PAGE staining 2 ug Liver TRAP1.

References

ab123775 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab123775.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up