Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
      P54274-2
    • Species
      Human
    • Molecular weight
      77 kDa including tags
    • Amino acids
      1 to 419

Specifications

Our Abpromise guarantee covers the use of ab116406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF-TOF
  • Purity
    > 90 % SDS-PAGE.

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.40
    Constituent: 99% PBS

General Info

  • Alternative names
    • hTRF1 AS
    • NIMA interacting protein 2
    • NIMA-interacting protein 2
    • PIN 2
    • PIN2
    • t TRF1
    • Telomeric protein Pin2
    • Telomeric protein Pin2/TRF1
    • Telomeric repeat binding factor (NIMA interacting) 1
    • Telomeric repeat binding factor 1
    • Telomeric repeat binding protein 1
    • Telomeric repeat-binding factor 1
    • TERF 1
    • Terf1
    • TERF1_HUMAN
    • TRBF 1
    • TRBF1
    • TRF
    • TRF 1
    • TTAGGG repeat binding factor 1
    • TTAGGG repeat-binding factor 1
    see all
  • Function
    Binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
  • Tissue specificity
    Highly expressed and ubiquitous. Isoform Pin2 predominates.
  • Sequence similarities
    Contains 1 HTH myb-type DNA-binding domain.
  • Domain
    The acidic N-terminal domain binds to the ankyrin repeats of TNKS1 and TNKS2. The C-terminal domain binds microtubules.
    The TRFH dimerization region mediates the interaction with TINF2.
  • Post-translational
    modifications
    Phosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage.
    ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA.
    Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.
  • Cellular localization
    Nucleus. Cytoplasm > cytoskeleton > spindle. Chromosome > telomere. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Associates with the mitotic spindle.
  • Information by UniProt

Images

  • Western blot - TRF1 protein (ab116406)
    Western blot - TRF1 protein (ab116406)
    10% SDS-PAGE stained with Coomassie Blue (Lane 1), immunobloting with anti-6xHis (Lane 2)

References

ab116406 has not yet been referenced specifically in any publications.

Publishing research using ab116406? Please let us know so that we can cite the reference in this datasheet.

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