Recombinant Human TRF1 protein (ab116406)

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Western blot - Recombinant Human TRF1 protein (ab116406)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Suitable for: WB, ELISA, SDS-PAGE, MS

    Description

    • Product name

      Recombinant Human TRF1 protein

    • Purity

      > 90 % SDS-PAGE.

    • Expression system

      Escherichia coli

    • Accession

      P54274-2

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Predicted molecular weight

        77 kDa including tags

      • Amino acids

        1 to 419

    Specifications

    Our Abpromise guarantee covers the use of ab116406 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Western blot

      ELISA

      SDS-PAGE

      Mass Spectrometry

    • Mass spectrometry

      MALDI-TOF-TOF

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 7.40
      Constituent: 99% PBS

    General Info

    • Alternative names

      • hTRF1 AS
      • NIMA interacting protein 2
      • NIMA-interacting protein 2
      • PIN 2
      • PIN2
      • t TRF1
      • Telomeric protein Pin2
      • Telomeric protein Pin2/TRF1
      • Telomeric repeat binding factor (NIMA interacting) 1
      • Telomeric repeat binding factor 1
      • Telomeric repeat binding protein 1
      • Telomeric repeat-binding factor 1
      • TERF 1
      • Terf1
      • TERF1_HUMAN
      • TRBF 1
      • TRBF1
      • TRF
      • TRF 1
      • TTAGGG repeat binding factor 1
      • TTAGGG repeat-binding factor 1
      see all

    • Function

      Binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.

    • Tissue specificity

      Highly expressed and ubiquitous. Isoform Pin2 predominates.

    • Sequence similarities

      Contains 1 HTH myb-type DNA-binding domain.

    • Domain

      The acidic N-terminal domain binds to the ankyrin repeats of TNKS1 and TNKS2. The C-terminal domain binds microtubules.
      The TRFH dimerization region mediates the interaction with TINF2.

    • Post-translational
      modifications

      Phosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage.
      ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA.
      Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.

    • Cellular localization

      Nucleus. Cytoplasm > cytoskeleton > spindle. Chromosome > telomere. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Associates with the mitotic spindle.
    • Information by UniProt

    Images

    • Western blot - Recombinant Human TRF1 protein (ab116406)
      Western blot - Recombinant Human TRF1 protein (ab116406)
      10% SDS-PAGE stained with Coomassie Blue (Lane 1), immunobloting with anti-6xHis (Lane 2)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab116406? Please let us know so that we can cite the reference in this datasheet.

    ab116406 has not yet been referenced specifically in any publications.

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