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    recombinant-human-trf2-protein-ab152737.pdf

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Epigenetics and Nuclear Signaling Chromosome Structure Telomeres
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Recombinant Human TRF2 protein (ab152737)

  • Datasheet
  • SDS
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SDS-PAGE - Recombinant Human TRF2 protein (ab152737)

    Key features and details

    • Expression system: Wheat germ
    • Suitable for: WB, ELISA, SDS-PAGE

    Description

    • Product name

      Recombinant Human TRF2 protein
    • Expression system

      Wheat germ
    • Accession

      Q15554-2
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        MAGGGGSSDGSGRAAGRRASRSSGRARRGRHEPGLGGPAERGAGEARLEE AVNRWVLKFYFHEALRAFRGSRYGDFRQIRDIMQALLVRPLGKEHTVSRL LRVMQCLSRIEEGENLDCSFDMEAELTPLESAINVLEMIKTEFTLTEAVV ESSRKLVKEAAVIICIKNKEFEKASKILKKHMSKDPTTQKLRNDLLNIIR EKNLAHPVIQNFSYETFQQKMLRFLESHLDDAEPYLLTVRLGPSPITMVC P
      • Predicted molecular weight

        55 kDa including tags
      • Amino acids

        1 to 251

    Specifications

    Our Abpromise guarantee covers the use of ab152737 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Western blot

      ELISA

      SDS-PAGE

    • Form

      Liquid
    • Additional notes

       

       

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.31% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • Telomeric DNA binding protein
      • Telomeric DNA-binding protein
      • Telomeric repeat binding factor 2
      • Telomeric repeat binding protein 2
      • Telomeric repeat-binding factor 2
      • TERF 2
      • Terf2
      • TERF2_HUMAN
      • TRBF 2
      • TRBF2
      • TRF 2
      • TRF2
      • TTAGGG repeat binding factor 2
      • TTAGGG repeat-binding factor 2
      see all
    • Function

      Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.
    • Tissue specificity

      Ubiquitous. Highly expressed in spleen, thymus, prostate, uterus, testis, small intestine, colon and peripheral blood leukocytes.
    • Sequence similarities

      Contains 1 HTH myb-type DNA-binding domain.
    • Domain

      The TRFH dimerization region mediates the interaction with DCLRE1B/Apollo but not TINF2.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus. Chromosome > telomere. Colocalizes with telomeric DNA in interphase cells and is located at chromosome ends during metaphase.
    • Target information above from: UniProt accession Q15554 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human TRF2 protein (ab152737)
      SDS-PAGE - Recombinant Human TRF2 protein (ab152737)
      12.5% SDS-PAGE analysis of ab152737 stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab152737? Please let us know so that we can cite the reference in this datasheet.

    ab152737 has been referenced in 1 publication.

    • Su CH  et al. An H2A Histone Isotype, H2ac, Associates with Telomere and Maintains Telomere Integrity. PLoS One 11:e0156378 (2016). WB . PubMed: 27228173

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