Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: MS, SDS-PAGE, WB
Product nameRecombinant Human Tri-ubiquitin (linkage-specific K48) protein
See all Tri-ubiquitin proteins and peptides
Purity> 95 % SDS-PAGE.
Expression systemEscherichia coli
Protein lengthFull length protein
Predicted molecular weight26 kDa
Our Abpromise guarantee covers the use of ab166765 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.32% Tris HCl, 0.03% EDTA, 0.88% Sodium chloride
FunctionUbiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
Sequence similaritiesBelongs to the ubiquitin family.
Contains 3 ubiquitin-like domains.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
All lanes : Anti-Ubiquitin mouse monoclonal
Lane 1 :
Recombinant Human Tri-ubiquitin (linkage-specific K48) protein (ab166765) at 0.1 µg
Lane 2 :
Recombinant Human Tri-ubiquitin (linkage-specific K48) protein (ab166765) at 0.01 µg
All lanes : Anti-mouse IgG-HRP
Developed using the ECL technique.
Ubiquitin chain mass was also verified by LC-MS.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab166765 has not yet been referenced specifically in any publications.