Recombinant Human Tri-ubiquitin (linkage-specific K48) protein (ab166765)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: MS, SDS-PAGE, WB
Description
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Product name
Recombinant Human Tri-ubiquitin (linkage-specific K48) protein
See all Tri-ubiquitin proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
26 kDa
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Specifications
Our Abpromise guarantee covers the use of ab166765 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Mass Spectrometry
SDS-PAGE
Western blot
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.32% Tris HCl, 0.03% EDTA, 0.88% Sodium chloride
General Info
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Alternative names
- Polyubiquitin-B
- RPS27A
- UBA52
see all -
Function
Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. -
Sequence similarities
Belongs to the ubiquitin family.
Contains 3 ubiquitin-like domains. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
Images
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All lanes : Anti-Ubiquitin mouse monoclonal
Lane 1 :Recombinant Human Tri-ubiquitin (linkage-specific K48) protein (ab166765) at 0.1 µg
Lane 2 :Recombinant Human Tri-ubiquitin (linkage-specific K48) protein (ab166765) at 0.01 µg
Secondary
All lanes : Anti-mouse IgG-HRP
Developed using the ECL technique.
Ubiquitin chain mass was also verified by LC-MS.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab166765 has not yet been referenced specifically in any publications.