Description

  • Product name

    Recombinant human TRIO protein (Active)
    See all TRIO proteins and peptides
  • Biological activity

    The specific activity of ab217836 was determined to be 1.6 nmol/min/mg.

  • Purity

    > 80 % SDS-PAGE.

  • Expression system

    Sf9 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      KKST SWHTALRLRK KSEKKDKDGK REGKLENGYR KSREGLSNKV SVKLLNPNYI YDVPPEFVIP LSEVTCETGE TVVLRCRVCG RPKASITWKG PEHNTLNNDG HYSISYSDLG EATLKIVGVT TEDDGIYTCI AVNDMGSASS SASLRVLGPG MDGIMVTWKD NFDSFYSEVA ELGRGRFSVV KKCDQKGTKR AVATKFVNKK LMKRDQVTHE LGILQSLQHP LLVGLLDTFE TPTSYILVLE MADQGRLLDC VVRWGSLTEG KIRAHLGEVL EAVRYLHNCR IAHLDLKPEN ILVDESLAKP TIKLADFGDA VQLNTTYYIH QLLGNPEFAA PEIILGNPVS LTSDTWSVGV LTYVLLSGVS PFLDDSVEET CLNICRLDFS FPDDYFKGVS QKAKEFVCFL LQEDPAKRPS AALALQEQWL QAGNGRSTGV LDTSRLTSFI ERRKHQNDVR PIRSIKNFLQ SRLLPRV
    • Predicted molecular weight

      82 kDa including tags
    • Amino acids

      2627 to 3097
    • Tags

      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab217836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.5
    Constituents: 25% Glycerol, 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.002% EDTA, 0.003% DTT, 0.002% PMSF

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • ARHGEF23
    • MEBAS
    • MRD44
    • PTPRF-interacting protein
    • tgat
    • trio
    • Trio Rho guanine nucleotide exchange factor
    • TRIO_HUMAN
    • Triple functional domain (PTPRF interacting)
    • Triple functional domain protein
    see all
  • Function

    Promotes the exchange of GDP by GTP. Together with leukocyte antigen-related (LAR) protein, it could play a role in coordinating cell-matrix and cytoskeletal rearrangements necessary for cell migration and cell growth.
  • Tissue specificity

    Highly expressed in heart, skeletal muscle, brain, pancreas, placenta, liver, kidney and lung.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 CRAL-TRIO domain.
    Contains 2 DH (DBL-homology) domains.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 2 PH domains.
    Contains 1 protein kinase domain.
    Contains 2 SH3 domains.
    Contains 4 spectrin repeats.
  • Domain

    The N-terminal DBL/GEF domain specifically catalyzes nucleotide exchange for RAC1, leading to the activation of Jun kinase and the production of membrane ruffles. The second DBL/GEF domain is an exchange factor for rhoa and induces the formation of stress fibers.
  • Post-translational
    modifications

    Phosphorylated on serine residue(s).
  • Cellular localization

    Cytoplasm.
  • Information by UniProt

Images

  • The specific activity of TRIO (ab217836) was determined to be 1.6 nmol/min/mg as per activity assay protocol
  • SDS PAGE analysis of ab217836
  • SDS PAGE analysis of ab217836
  • Kinase activity plot. The specific activity was determined to be 1.6 nmol/min/mg.

  • The purity of ab217836 was determined to be >80% by densitometry. MW ~82 kDa.

References

ab217836 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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