Recombinant Human TRPV5 protein (ab153420)

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SDS-PAGE - Recombinant Human TRPV5 protein (ab153420)

    Key features and details

    • Expression system: Wheat germ
    • Tags: GST tag N-Terminus
    • Suitable for: WB, ELISA

    Description

    • Product name

      Recombinant Human TRPV5 protein

    • Expression system

      Wheat germ

    • Protein length

      Protein fragment

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGGFLPKAEGPGSQLQKLLPSFLVREQDWDQHLDKLHMLQQKRILESPLL RASKENDLSVLRQLLLDCTCDVRQRGALGETALHIAALYDNLEAALVLME

      • Amino acids

        1 to 100

      • Tags

        GST tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab153420 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Western blot

      ELISA

    • Form

      Liquid

    • Additional notes

       

       

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.31% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • Calcium transport protein 2
      • Calcium transporter 2
      • CAT 2
      • CAT2
      • ECaC
      • ECAC 1
      • ECaC1
      • Epithelial calcium channel 1
      • Osm 9 like TRP channel 3
      • Osm-9-like TRP channel 3
      • OTRPC 3
      • OTRPC3
      • Transient receptor potential cation channel subfamily V member 5
      • TRPV 5
      • TrpV5
      • TRPV5_HUMAN
      see all

    • Function

      Constitutively active calcium selective cation channel thought to be involved in Ca(2+) reabsorption in kidney and intestine. The channel is activated by low internal calcium level and the current exhibits an inward rectification. A Ca(2+)-dependent feedback regulation includes fast channel inactivation and slow current decay. Heteromeric assembly with TRPV6 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating.

    • Tissue specificity

      Expressed at high levels in kidney, small intestine and pancreas, and at lower levels in testis, prostate, placenta, brain, colon and rectum.

    • Sequence similarities

      Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV5 sub-subfamily.
      Contains 5 ANK repeats.

    • Post-translational
      modifications

      Glycosylated.

    • Cellular localization

      Apical cell membrane. Colocalized with S100A10 and ANAX2 along the apical domain of kidney distal tubular cells (By similarity). The expression of the glycosylated form in the cell membrane is increased in the presence of WNK3.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human TRPV5 protein (ab153420)
      SDS-PAGE - Recombinant Human TRPV5 protein (ab153420)
      ab153420 on a 12.5% SDS-PAGE stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab153420? Please let us know so that we can cite the reference in this datasheet.

    ab153420 has not yet been referenced specifically in any publications.

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