Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human Tryptophan Hydroxylase/TPH protein (Active)
Assay Conditions: Reaction was performed at room temperature in assay buffer containing 50 mM MOPS, pH 7.0, 60 μM tryptophan, 100 mM ammonium sulfate, 100 μM ferrous ammonium sulfate, 0.5 mM TCEP, 0.3 mM 6-methyl tetrahydropterin, 0.05 mg/ml catalase, 1 mM DTT and TPH1. Fluorescence signal was measured at Em360nm/Ex300nm.
Purity> 90 % SDS-PAGE.
Expression systemEscherichia coli
Protein lengthProtein fragment
SequenceMHHHHHHVPWFPKKISDLDHCANRVLMYGSELDADHPGFKDNVYRKRRKY FADLAMNYKHGDPIPKVEFTEEEIKTWGTVFQELNKLYPTHACREYLKNL PLLSKYCGYREDNIPQLEDVSNFLKERTGFSIRPVAGYLSPRDFLSGLAF RVFHCTQYVRHSSDPFYTPEPDTCHELLGHVPLLAEPSFAQFSQEIGLAS LGASEEAVQKLATCYFFTVEFGLCKQDGQLRVFGAGLLSSISELKHALSG HAKVKPFDPKITCKQECLITTFQDVYFVSESFEDAKEKMREFTKTIKRPF GVKYNPYTRSIQILKDTKSITSA
Predicted molecular weight37 kDa including tags
Amino acids105 to 420
TagsHis tag N-Terminus
Our Abpromise guarantee covers the use of ab206448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product was previously labelled as Tryptophan Hydroxylase
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Preservative: 1.16% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Indoleacetic acid 5 hydroxylase
- L tryptophan hydroxylase
Tissue specificityIsoform 2 seems to be less widely expressed than isoform 1.
PathwayAromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
Sequence similaritiesBelongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Contains 1 ACT domain.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab206448 has not yet been referenced specifically in any publications.