Recombinant human Tryptophan Hydroxylase/TPH protein (Active) (ab206448)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human Tryptophan Hydroxylase/TPH protein (Active) -
Biological activity
Assay Conditions: Reaction was performed at room temperature in assay buffer containing 50 mM MOPS, pH 7.0, 60 μM tryptophan, 100 mM ammonium sulfate, 100 μM ferrous ammonium sulfate, 0.5 mM TCEP, 0.3 mM 6-methyl tetrahydropterin, 0.05 mg/ml catalase, 1 mM DTT and TPH1. Fluorescence signal was measured at Em360nm/Ex300nm.
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Purity
> 90 % SDS-PAGE.
Affinity purified. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MHHHHHHVPWFPKKISDLDHCANRVLMYGSELDADHPGFKDNVYRKRRKY FADLAMNYKHGDPIPKVEFTEEEIKTWGTVFQELNKLYPTHACREYLKNL PLLSKYCGYREDNIPQLEDVSNFLKERTGFSIRPVAGYLSPRDFLSGLAF RVFHCTQYVRHSSDPFYTPEPDTCHELLGHVPLLAEPSFAQFSQEIGLAS LGASEEAVQKLATCYFFTVEFGLCKQDGQLRVFGAGLLSSISELKHALSG HAKVKPFDPKITCKQECLITTFQDVYFVSESFEDAKEKMREFTKTIKRPF GVKYNPYTRSIQILKDTKSITSA -
Predicted molecular weight
37 kDa including tags -
Amino acids
105 to 420 -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab206448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Additional notes
This product was previously labelled as Tryptophan Hydroxylase
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 1.16% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Indoleacetic acid 5 hydroxylase
- L tryptophan hydroxylase
- MGC119994
see all -
Tissue specificity
Isoform 2 seems to be less widely expressed than isoform 1. -
Pathway
Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2. -
Sequence similarities
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Contains 1 ACT domain. - Information by UniProt
Images
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Functional Studies - Recombinant human Tryptophan Hydroxylase/TPH protein (Active) (ab206448)
Activity assay using ab206448
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SDS-PAGE - Recombinant human Tryptophan Hydroxylase/TPH protein (Active) (ab206448)6-20% SDS-PAGE stained with Coomassie Blue.
Lane 1: ab206448 (5 µg)
Lane 2: Protein Marker
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab206448 has not yet been referenced specifically in any publications.