Key features and details
- Expression system: Escherichia coli
- Purity: > 85% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
Product nameRecombinant Human Tryptophanyl tRNA synthetase/WRS protein (Tagged)
See all Tryptophanyl tRNA synthetase/WRS proteins and peptides
Purity> 85 % SDS-PAGE.
Expression systemEscherichia coli
Protein lengthFull length protein
SequencePNSEPASLLELFNSIATQGELVRSLKAGNASKDEIDSAVKMLVSLKMSYK AAAGEDYKADCPPGNPAPTSNHGPDATEAEEDFVDPWTVQTSSAKGIDYD KLIVRFGSSKIDKELINRIERATGQRPHHFLRRGIFFSHRDMNQVLDAYE NKKPFYLYTGRGPSSEAMHVGHLIPFIFTKWLQDVFNVPLVIQMTDDEKY LWKDLTLDQAYSYAVENAKDIIACGFDINKTFIFSDLDYMGMSSGFYKNV VKIQKHVTFNQVKGIFGFTDSDCIGKISFPAIQAAPSFSNSFPQIFRDRT DIQCLIPCAIDQDPYFRMTRDVAPRIGYPKPALLHSTFFPALQGAQTKMS ASDPNSSIFLTDTAKQIKTKVNKHAFSGGRDTIEEHRQFGGNCDVDVSFM YLTFFLEDDDKLEQIRKDYTSGAMLTGELKKALIEVLQPLIAEHQARRKE VTDEIVKEFMTPRKLSFDFQ
Predicted molecular weight60 kDa including tags
Amino acids2 to 471
TagsHis tag N-Terminus
Additional sequence informationN-terminal 10xHis-tagged and C-terminal Myc-tagged.
- Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-Myc tag antibody [Myc.A7] (ab18185)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-Myc tag antibody (ab9106)
- Anti-6X His tag® antibody (ab9108)
- Anti-Myc tag antibody - ChIP Grade (ab9132)
- Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733)
Our Abpromise guarantee covers the use of ab236161 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Previously labelled as Tryptophanyl tRNA synthetase.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
- Gamma 2
FunctionIsoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.
Sequence similaritiesBelongs to the class-I aminoacyl-tRNA synthetase family.
Contains 1 WHEP-TRS domain.
modificationsProteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236161 has not yet been referenced specifically in any publications.