Recombinant Human Tryptophanyl tRNA synthetase/WRS protein (Tagged) (ab236161)
Key features and details
- Expression system: Escherichia coli
- Purity: > 85% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human Tryptophanyl tRNA synthetase/WRS protein (Tagged)
See all Tryptophanyl tRNA synthetase/WRS proteins and peptides -
Purity
> 85 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
PNSEPASLLELFNSIATQGELVRSLKAGNASKDEIDSAVKMLVSLKMSYK AAAGEDYKADCPPGNPAPTSNHGPDATEAEEDFVDPWTVQTSSAKGIDYD KLIVRFGSSKIDKELINRIERATGQRPHHFLRRGIFFSHRDMNQVLDAYE NKKPFYLYTGRGPSSEAMHVGHLIPFIFTKWLQDVFNVPLVIQMTDDEKY LWKDLTLDQAYSYAVENAKDIIACGFDINKTFIFSDLDYMGMSSGFYKNV VKIQKHVTFNQVKGIFGFTDSDCIGKISFPAIQAAPSFSNSFPQIFRDRT DIQCLIPCAIDQDPYFRMTRDVAPRIGYPKPALLHSTFFPALQGAQTKMS ASDPNSSIFLTDTAKQIKTKVNKHAFSGGRDTIEEHRQFGGNCDVDVSFM YLTFFLEDDDKLEQIRKDYTSGAMLTGELKKALIEVLQPLIAEHQARRKE VTDEIVKEFMTPRKLSFDFQ -
Predicted molecular weight
60 kDa including tags -
Amino acids
2 to 471 -
Tags
His tag N-Terminus -
Additional sequence information
N-terminal 10xHis-tagged and C-terminal Myc-tagged.
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Associated products
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Related Products
- Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423] (ab109213)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-Myc tag antibody [Myc.A7] (ab18185)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-Myc tag antibody (ab9106)
- Anti-6X His tag® antibody (ab9108)
- Anti-Myc tag antibody - ChIP Grade (ab9132)
- Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733)
Specifications
Our Abpromise guarantee covers the use of ab236161 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Additional notes
Previously labelled as Tryptophanyl tRNA synthetase.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.2
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- Gamma 2
- GAMMA-2
- Gamma2
see all -
Function
Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression. -
Sequence similarities
Belongs to the class-I aminoacyl-tRNA synthetase family.
Contains 1 WHEP-TRS domain. -
Post-translational
modificationsProteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS. -
Cellular localization
Cytoplasm. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab236161 has not yet been referenced specifically in any publications.