Product nameRecombinant human TTK/Mps1 protein
See all TTK/Mps1 proteins and peptides
Protein lengthFull length protein
SourceBaculovirus infected Sf9 cells
Amino Acid Sequence
TagsGST tag N-Terminus
Our Abpromise guarantee covers the use of ab89589 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityThe Specific activity of ab89589 was determined to be 19 nmol/min/mg.
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- cancer/testis antigen 96
- Dual specificity protein kinase TTK
FunctionPhosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint.
Tissue specificityPresent in rapidly proliferating cell lines.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
- Information by UniProt
SDS-PAGE showing ab89589 at approximately 130kDa.
Kinase Assay demonstrating specific activity of ab89589.
Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108) at 10 µg/ml +
Recombinant human TTK/Mps1 protein (ab89589) at 0.01 µg
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 8 minutes
ab11108 recognizes the full length tagged recombinant TTK/Mps1 protein (ab89589) which has an expected molecular weight of 130 kDa.
ab89589 has not yet been referenced specifically in any publications.