Recombinant Human TWF1/Twinfilin-1 protein (ab116148)

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Description

  • Product name

    Recombinant Human TWF1/Twinfilin-1 protein
    See all TWF1/Twinfilin-1 proteins and peptides

  • Purity

    > 80 % SDS-PAGE.
    purified by using conventional chromatography

  • Expression system

    Escherichia coli

  • Accession

    Q12792

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MGSSHHHHHH SSGLVPRGSH MGSHM MLYAA TRATLKKEFG GGHIKDEVFG TVKEDVSLHG YKKYLLSQSS PAPLTAAEEE LRQIKINEVQ TDVGVDTKHQ TLQGVAFPIS REAFQALEKL NNRQLNYVQL EIDIKNEIII LANTTNTELK DLPKRIPKDS ARYHFFLYKH SHEGDYLESI VFIYSMPGYT CSIRERMLYS SCKSRLLEIV ERQLQMDVIR KIEIDNGDEL TADFLYEEVH PKQHAHKQSF AKPKGPAGKR GIRRLIRGPA ETEATTD

    • Predicted molecular weight

      32 kDa including tags

    • Amino acids

      100 to 350

    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab116148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Mass spectrometry

    MALDI-TOF

  • Form

    Liquid

  • Additional notes

     This product was previously labelled as TWF1

     

     This product was previously labelled as TWF1

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 40% Glycerol, 0.88% Sodium chloride, 0.02% DTT

General Info

  • Alternative names

    • A6
    • A6 protein tyrosine kinase
    • MGC105817
    • MGC23788
    • MGC41876
    • Protein A6
    • Protein tyrosine kinase 9
    • PTK9
    • PTK9 protein tyrosine kinase 9
    • TWF 1
    • twf1
    • TWF1_HUMAN
    • Twinfilin
    • Twinfilin 1
    • Twinfilin actin binding protein, homolog 1
    • Twinfilin, actin-binding protein, homolog 1 (Drosophila)
    • Twinfilin, Drosophila, homolog of, 1
    • Twinfilin-1
    see all

  • Function

    Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles.

  • Tissue specificity

    Expressed at high levels in the colon, testis, ovary, prostate and lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver.

  • Sequence similarities

    Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.
    Contains 2 ADF-H domains.

  • Post-translational
    modifications

    Phosphorylated on serine and threonine residues.

  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton. Diffuse cytoplasmic localization with perinuclear and G-actin-rich cortical actin structures sublocalization. Also found at membrane ruffles and cell-cell contacts.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human TWF1/Twinfilin-1 protein (ab116148)
    SDS-PAGE - Recombinant Human TWF1/Twinfilin-1 protein (ab116148)
    15% SDS-PAGE showing ab116148 at approximately 31.5kDa (3µg)

References

ab116148 has not yet been referenced specifically in any publications.

Publishing research using ab116148? Please let us know so that we can cite the reference in this datasheet.

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