Recombinant Human TWF1/Twinfilin-1 protein (ab116148)

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SDS-PAGE - Recombinant Human TWF1/Twinfilin-1 protein (ab116148)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 80% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Recombinant Human TWF1/Twinfilin-1 protein
      See all TWF1/Twinfilin-1 proteins and peptides

    • Purity

      > 80 % SDS-PAGE.
      purified by using conventional chromatography

    • Expression system

      Escherichia coli

    • Accession

      Q12792

    • Protein length

      Protein fragment

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHH SSGLVPRGSH MGSHM MLYAA TRATLKKEFG GGHIKDEVFG TVKEDVSLHG YKKYLLSQSS PAPLTAAEEE LRQIKINEVQ TDVGVDTKHQ TLQGVAFPIS REAFQALEKL NNRQLNYVQL EIDIKNEIII LANTTNTELK DLPKRIPKDS ARYHFFLYKH SHEGDYLESI VFIYSMPGYT CSIRERMLYS SCKSRLLEIV ERQLQMDVIR KIEIDNGDEL TADFLYEEVH PKQHAHKQSF AKPKGPAGKR GIRRLIRGPA ETEATTD

      • Predicted molecular weight

        32 kDa including tags

      • Amino acids

        100 to 350

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab116148 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Mass spectrometry

      MALDI-TOF

    • Form

      Liquid

    • Additional notes

       This product was previously labelled as TWF1

       

       This product was previously labelled as TWF1

       

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.02% DTT, 0.32% Tris HCl, 40% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    General Info

    • Alternative names

      • A6
      • A6 protein tyrosine kinase
      • MGC105817
      • MGC23788
      • MGC41876
      • Protein A6
      • Protein tyrosine kinase 9
      • PTK9
      • PTK9 protein tyrosine kinase 9
      • TWF 1
      • twf1
      • TWF1_HUMAN
      • Twinfilin
      • Twinfilin 1
      • Twinfilin actin binding protein, homolog 1
      • Twinfilin, actin-binding protein, homolog 1 (Drosophila)
      • Twinfilin, Drosophila, homolog of, 1
      • Twinfilin-1
      see all

    • Function

      Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles.

    • Tissue specificity

      Expressed at high levels in the colon, testis, ovary, prostate and lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver.

    • Sequence similarities

      Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.
      Contains 2 ADF-H domains.

    • Post-translational
      modifications

      Phosphorylated on serine and threonine residues.

    • Cellular localization

      Cytoplasm. Cytoplasm > cytoskeleton. Diffuse cytoplasmic localization with perinuclear and G-actin-rich cortical actin structures sublocalization. Also found at membrane ruffles and cell-cell contacts.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human TWF1/Twinfilin-1 protein (ab116148)
      SDS-PAGE - Recombinant Human TWF1/Twinfilin-1 protein (ab116148)
      15% SDS-PAGE showing ab116148 at approximately 31.5kDa (3µg)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab116148? Please let us know so that we can cite the reference in this datasheet.

    ab116148 has not yet been referenced specifically in any publications.

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