Recombinant human TXNRD1 protein (ab168011)

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SDS-PAGE - Recombinant human TXNRD1 protein (ab168011)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Active: Yes
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, Functional Studies

    Description

    • Product name

      Recombinant human TXNRD1 protein
      See all TXNRD1 proteins and peptides

    • Biological activity

      Specific activity is 0.48-0.60 units/ml, and was measured in a coupled assay with DTNB and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412 nm.

    • Purity

      > 95 % SDS-PAGE.

    • Expression system

      Escherichia coli

    • Accession

      Q16881

    • Protein length

      Protein fragment

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHH SSGLVPRGSH MYDYDLIIIG GGSGGLAAAK EAAQYGKKVM VLDFVTPTPL GTRWGLGGTC VNVGCIPKKL MHQAALLGQA LQDSRNYGWK VEETVKHDWD RMIEAVQNHI GSLNWGYRVA LREKKVVYEN AYGQFIGPHR IKATNNKGKE KIYSAERFLI ATGERPRYLG IPGDKEYCIS SDDLFSLPYC PGKTLVVGAS YVALECAGFL AGIGLDVTVM VRSILLRGFD QDMANKIGEH MEEHGIKFIR QFVPIKVEQI EAGTPGRLRV VAQSTNSEEI IEGEYNTVML AIGRDACTRK IGLETVGVKI NEKTGKIPVT DEEQTNVPYI YAIGDILEDK VELTPVAIQA GRLLAQRLYA GSTVKCDYEN VPTTVFTPLE YGACGLSEEK AVEKFGEENI EVYHSYFWPL EWTIPSRDNN KCYAKIICNT KDNERVVGFH VLGPNAGEVT QGFAAALKCG LTKKQLDSTI GIHPVCAEVF TTLSVTKRSG ASILQAGC

      • Predicted molecular weight

        56 kDa including tags

      • Amino acids

        161 to 647

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab168011 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Functional Studies

    • Form

      Liquid

    • Additional notes

      Centrifuge the vial prior to opening
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.40
      Constituents: 90% PBS, 10% Glycerol

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    General Info

    • Alternative names

      • cytoplasmic
      • Gene associated with retinoic and IFN-induced mortality 12 protein
      • Gene associated with retinoic and interferon-induced mortality 12 protein
      • Gene associated with retinoid IFN induced mortality 12 protein
      • GRIM 12
      • GRIM-12
      • GRIM12
      • KDRF
      • KM 102 derived reductase like factor
      • KM-102-derived reductase-like factor
      • MGC9145
      • Oxidoreductase
      • Thioredoxin reductase 1
      • Thioredoxin reductase 1 cytoplasmic
      • Thioredoxin reductase GRIM 12
      • Thioredoxin reductase TR1
      • TR
      • TR 1
      • TR1
      • TRXR 1
      • TRXR1
      • TRXR1_HUMAN
      • TXNR
      • TXNRD 1
      • Txnrd1
      see all

    • Function

      Isoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid.

    • Tissue specificity

      Isoform 1 is expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver.

    • Sequence similarities

      Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
      Contains 1 glutaredoxin domain.

    • Domain

      The N-terminal glutaredoxin domain found in isoform 1 does not contain the C-P-Y-C redox-active motif normally found in glutaredoxins and has been found to be inactive in classical glutaredoxin assays.

    • Post-translational
      modifications

      The N-terminus of isoform 5 is blocked.
      ISGylated.

    • Cellular localization

      Cytoplasm and Cytoplasm. Nucleus.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant human TXNRD1 protein (ab168011)
      SDS-PAGE - Recombinant human TXNRD1 protein (ab168011)
      15% SDS-PAGE analysis of 3 µg ab168011.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab168011? Please let us know so that we can cite the reference in this datasheet.

    ab168011 has been referenced in 1 publication.

    • Wang X  et al. Peperomin E and its orally bioavailable analog induce oxidative stress-mediated apoptosis of acute myeloid leukemia progenitor cells by targeting thioredoxin reductase. Redox Biol 24:101153 (2019). PubMed: 30909158

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