Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His-T7 tag N-Terminus
- Suitable for: SDS-PAGE
Product nameRecombinant Human Tyrosyl tRNA synthetase/TyrRS protein (Tagged)
See all Tyrosyl tRNA synthetase/TyrRS proteins and peptides
Purity> 90 % SDS-PAGE.
The final product was refolded using unique “temperature shift inclusion body refolding” technology and chromatographically purified.
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMASMTGGQQMGRGHHHHHHENLYFQGGEFGDAPSPEEKLHLITRNLQEVL GEEKLKEILKERELKIYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTIL FADLHAYLDNMKAPWELLELRVSYYENVIKAMLESIGVPLEKLKFIKGTD YQLSKEYTLDVYRLSSVVTQHDSKKAGAEVVKQVEHPLLSGLLYPGLQAL DEEYLKVDAQFGGIDQRKIFTFAEKYLPALGYSKRVHLMNPMVPGLTGSK MSSSEEESKIDLLDRKEDVKKKLKKAFCEPGNVENNGVLSFIKHVLFPLK SEFVILRDEKWGGNKTYTAYVDLEKDFAAEVVHPGDLKNSVEVALNKLLD PIREKFNTPALKKLASAAYPDPSKQKPMAKGPAKNSEPEEVIPSRLDIRV GKIITVEKHPDADSLYVEKIDVGEAEPRTVVSGLVQFVPKEELQDRLVVV LCNLKPQKMRGVESQGMLLCASIEGINRQVEPLDPPAGSAPGEHVFVKGY EKGQPDEELKPKKKVFEKLQADFKISEECIAQWKQTNFMTKLGSISCKSL KGGNIS
Predicted molecular weight62 kDa including tags
Amino acids2 to 528
TagsHis-T7 tag N-Terminus
Additional sequence informationConstructed with codon optimization and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal. NP_003671.1.
- Anti-Tyrosyl tRNA synthetase/TyrRS antibody [EPR9926(B)] (ab150429)
- Anti-TEV Tag antibody [KT88] (ab179875)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-Tyrosyl tRNA synthetase/TyrRS antibody (ab31535)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08] (ab50961)
- Anti-6X His tag® antibody (ab9108)
- Anti-T7 tag® antibody (ab9115)
Our Abpromise guarantee covers the use of ab190402 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Previously labelled as Tyrosyl tRNA synthetase
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituent: 0.32% Tris HCl
Contains NACl, EDTA, KCl, arginine, DTT and glycerol
- Tyrosine tRNA ligase, cytoplasmic
FunctionCatalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).
Involvement in diseaseDefects in YARS are the cause of Charcot-Marie-Tooth disease dominant intermediate type C (CMTDIC) [MIM:608323]. CMTDIC is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.
Sequence similaritiesBelongs to the class-I aminoacyl-tRNA synthetase family.
Contains 1 tRNA-binding domain.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab190402 has not yet been referenced specifically in any publications.