The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
>90% by SDS-PAGE.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00 Constituents: 2.4% Urea, 0.32% Tris HCl, 10% Glycerol
Human U2 snRNP auxiliary factor small subunit9
Splicing factor U2AF 35 kDa subunit
U2 auxiliary factor 35 kDa subunit
U2 small nuclear ribonucleoprotein auxillary factor 35 KD subunit
U2 small nuclear RNA auxiliary factor 1
U2 snRNP auxiliary factor small subunit
U2(RNU2) small nuclear RNA auxiliary factor 1
U2(RNU2) small nuclear RNA auxiliary factor binding protein
Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron.
Belongs to the splicing factor SR family. Contains 2 C3H1-type zinc fingers. Contains 1 RRM (RNA recognition motif) domain.
The C-terminal SR-rich domain is required for interactions with SR proteins and the splicing regulators TRA and TRA2, and the N-terminal domain is required for formation of the U2AF1/U2AF2 heterodimer.
Phosphorylated upon DNA damage, probably by ATM or ATR.