The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.
>95% by SDS-PAGE .
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Monocyte protein 4
UBA6 ubiquitin activating enzyme E1
Ubiquitin activating enzyme 6
Ubiquitin activating enzyme E1 like 2
Ubiquitin activating enzyme E1 like protein 2
Ubiquitin like modifier activating enzyme 6
Ubiquitin-activating enzyme 6
Ubiquitin-activating enzyme E1-like protein 2
Ubiquitin-like modifier-activating enzyme 6
Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Required for UBD/FAT10 conjugation. Isoform 2 may play a key role in ubiquitin system and may influence spermatogenesis and male fertility.
Widely expressed. Isoform 2 is predominantly expressed in testis with higher expression in adult testis than in fetal testis.