Recombinant human UBC13 protein (Active) (ab269107)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human UBC13 protein (Active) -
Biological activity
The specific activity of ab269107 was determined to be 9 nmol/min/mg in a ubiquitination assay using wild-type ubiquitin protein substrate.
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Purity
> 95 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
AGLPRRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGT FKLELFLPEEYPMAAPKVRFMTKIYHPNVDKLGRICLDILKDKWSPALQI RTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIETARAWTRLYAMNN I -
Molecular weight information
SDS-PAGE molecular weight: 18 kDa -
Amino acids
2 to 152 -
Tags
His tag N-Terminus -
Additional sequence information
GenBank: NM_003348
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab269107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.00
Preservative: 1.02% Imidazole
Constituents: 0.82% Sodium phosphate, 1.74% Sodium chloride, 0.002% PMSF, 0.004% DTT, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Bendless like ubiquitin conjugating enzyme
- BLU
- Human epidermoid carcinoma mRNA for ubiquitin conjugating enzyme E2
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Relevance
UBC13 is a member of the E2 ubiquitin-conjugating enzyme family. It interacts with UBE2V2 to form a heterodimer that catalyzes the synthesis of poly-ubiquitin chains that are linked through 'Lys 63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. UBC13 may play a role in the control of progress through the cell cycle and differentiation by mediating transcriptional activation of target genes. UBC13 plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. UBC13 acts with the E3 ligase SHPRH in the poly-ubiquitination of PCNA during genotoxic stress, which is required for DNA repair. It also interacts with ZNRF2. -
Cellular localization
Nuclear
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab269107 has not yet been referenced specifically in any publications.