Recombinant Human UBC6e protein (Tagged) (ab177256)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% Densitometry
- Tags: proprietary tag N-Terminus
- Suitable for: WB, SDS-PAGE
Description
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Product name
Recombinant Human UBC6e protein (Tagged)
See all UBC6e proteins and peptides -
Purity
> 70 % Densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
METRYNLKSPAVKRLMKEAAELKDPTDHYHAQPLEDNLFEWHFTVRGPPD SDFDGGVYHGRIVLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPE TWQPSWSIRTALLAIIGFMPTKGEGAIGSLDYTPEERRALAKKSQDFCCE GCGSAMKDVLLPLKSGSDSSQADQEAKELARQISFKAEVNSSGKTISESD LNHSFSLTDLQDDIPTTFQGATASTSYGLQNSSAASFHQPTQPVAKNTSM SPRQRRAQQQSQRRLSTSPDVIQGHQPRDNHTDHGGSAVLIVILTLALAA LIFRRIYLANEYIFDFEL -
Predicted molecular weight
70 kDa including tags -
Amino acids
1 to 318 -
Tags
proprietary tag N-Terminus
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Specifications
Our Abpromise guarantee covers the use of ab177256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
SDS-PAGE
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Form
Liquid -
Additional notes
It is recommended to store the protein at -80°C in glycerol to preserve the activity the protein.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.79% Tris HCl, 0.29% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- CGI-76
- HSPC153
- HSPC205
see all -
Function
Catalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab177256 has not yet been referenced specifically in any publications.