Recombinant Human UBC6e protein (Tagged) (ab177256)

Submit a review Submit a question

Description

  • Product name

    Recombinant Human UBC6e protein (Tagged)
    See all UBC6e proteins and peptides

  • Purity

    > 70 % Densitometry.

  • Expression system

    Baculovirus infected Sf9 cells

  • Accession

    Q9Y385

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      METRYNLKSPAVKRLMKEAAELKDPTDHYHAQPLEDNLFEWHFTVRGPPD SDFDGGVYHGRIVLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPE TWQPSWSIRTALLAIIGFMPTKGEGAIGSLDYTPEERRALAKKSQDFCCE GCGSAMKDVLLPLKSGSDSSQADQEAKELARQISFKAEVNSSGKTISESD LNHSFSLTDLQDDIPTTFQGATASTSYGLQNSSAASFHQPTQPVAKNTSM SPRQRRAQQQSQRRLSTSPDVIQGHQPRDNHTDHGGSAVLIVILTLALAA LIFRRIYLANEYIFDFEL

    • Predicted molecular weight

      70 kDa including tags

    • Amino acids

      1 to 318

    • Tags

      proprietary tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab177256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Form

    Liquid

  • Additional notes

    It is recommended to store the protein at -80°C in glycerol to preserve the activity the protein.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 0.79% Tris HCl, 0.29% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol

General Info

  • Alternative names

    • CGI-76
    • HSPC153
    • HSPC205
    • HSU93243
    • HSUBC6e
    • MGC12555
    • NCUBE-1
    • NCUBE1
    • Non-canonical ubiquitin-conjugating enzyme 1
    • UB2J1_HUMAN
    • UBC6
    • UBC6E
    • Ubc6p
    • Ube2j1
    • Ubiquitin conjugating enzyme E2, J1 (UBC6 homolog, yeast)
    • Ubiquitin conjugating enzyme E2, J1, U
    • Ubiquitin-conjugating enzyme E2 J1
    • Yeast ubiquitin-conjugating enzyme UBC6 homolog E
    see all

  • Function

    Catalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum.

  • Pathway

    Protein modification; protein ubiquitination.

  • Sequence similarities

    Belongs to the ubiquitin-conjugating enzyme family.

  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human UBC6e protein (Tagged) (ab177256)
    SDS-PAGE - Recombinant Human UBC6e protein (Tagged) (ab177256)

    SDS-PAGE analysis of ab177256.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References

Publishing research using ab177256? Please let us know so that we can cite the reference in this datasheet.

ab177256 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab177256.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com