Recombinant Human UBC6e protein (Tagged) (ab177256)

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SDS-PAGE - Recombinant Human UBC6e protein (Tagged) (ab177256)

    Key features and details

    • Expression system: Baculovirus infected Sf9 cells
    • Purity: > 70% Densitometry
    • Tags: proprietary tag N-Terminus
    • Suitable for: WB, SDS-PAGE

    Description

    • Product name

      Recombinant Human UBC6e protein (Tagged)
      See all UBC6e proteins and peptides

    • Purity

      > 70 % Densitometry.

    • Expression system

      Baculovirus infected Sf9 cells

    • Accession

      Q9Y385

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        METRYNLKSPAVKRLMKEAAELKDPTDHYHAQPLEDNLFEWHFTVRGPPD SDFDGGVYHGRIVLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPE TWQPSWSIRTALLAIIGFMPTKGEGAIGSLDYTPEERRALAKKSQDFCCE GCGSAMKDVLLPLKSGSDSSQADQEAKELARQISFKAEVNSSGKTISESD LNHSFSLTDLQDDIPTTFQGATASTSYGLQNSSAASFHQPTQPVAKNTSM SPRQRRAQQQSQRRLSTSPDVIQGHQPRDNHTDHGGSAVLIVILTLALAA LIFRRIYLANEYIFDFEL

      • Predicted molecular weight

        70 kDa including tags

      • Amino acids

        1 to 318

      • Tags

        proprietary tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab177256 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Western blot

      SDS-PAGE

    • Form

      Liquid

    • Additional notes

      It is recommended to store the protein at -80°C in glycerol to preserve the activity the protein.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on Dry Ice. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

      pH: 7.50
      Constituents: 0.79% Tris HCl, 0.29% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)

    General Info

    • Alternative names

      • CGI-76
      • HSPC153
      • HSPC205
      • HSU93243
      • HSUBC6e
      • MGC12555
      • NCUBE-1
      • NCUBE1
      • Non-canonical ubiquitin-conjugating enzyme 1
      • UB2J1_HUMAN
      • UBC6
      • UBC6E
      • Ubc6p
      • Ube2j1
      • Ubiquitin conjugating enzyme E2, J1 (UBC6 homolog, yeast)
      • Ubiquitin conjugating enzyme E2, J1, U
      • Ubiquitin-conjugating enzyme E2 J1
      • Yeast ubiquitin-conjugating enzyme UBC6 homolog E
      see all

    • Function

      Catalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum.

    • Pathway

      Protein modification; protein ubiquitination.

    • Sequence similarities

      Belongs to the ubiquitin-conjugating enzyme family.

    • Cellular localization

      Endoplasmic reticulum membrane.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human UBC6e protein (Tagged) (ab177256)
      SDS-PAGE - Recombinant Human UBC6e protein (Tagged) (ab177256)

      SDS-PAGE analysis of ab177256.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab177256? Please let us know so that we can cite the reference in this datasheet.

    ab177256 has not yet been referenced specifically in any publications.

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