Recombinant Human UBE2I / UBC9 protein (BSA and azide free) (ab173073)

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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Endotoxin level: < 1.000 Eu/µg
  • Tags: proprietary tag N-Terminus
  • Suitable for: HPLC, SDS-PAGE

Description

  • Product name

    Recombinant Human UBE2I / UBC9 protein (BSA and azide free)
    See all UBE2I / UBC9 proteins and peptides

  • Purity

    > 95 % SDS-PAGE.
    Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    Escherichia coli

  • Accession

    P63279

  • Protein length

    Full length protein

  • Animal free

    No

  • Carrier free

    Yes

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDLVPRGSHMSGIALSRLAQERKAWRKDHPFG FVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPP KCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEP NIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS

    • Predicted molecular weight

      44 kDa including tags

    • Amino acids

      1 to 158

    • Tags

      proprietary tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab173073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    HPLC

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 1.19% HEPES, 0.88% Sodium chloride

General Info

  • Alternative names

    • C358B7.1
    • p18
    • SUMO 1 protein ligase
    • SUMO conjugating enzyme UBC9
    • SUMO-conjugating enzyme UBC9
    • SUMO-protein ligase
    • SUMO1 protein ligase
    • UBC9
    • UBC9_HUMAN
    • UBCE9
    • Ube2i
    • Ubiquitin carrier protein
    • Ubiquitin carrier protein 9
    • Ubiquitin carrier protein I
    • Ubiquitin conjugating enzyme 9
    • Ubiquitin conjugating enzyme E2I (homologous to yeast UBC9)
    • Ubiquitin conjugating enzyme E2I (UBC9 homolog, yeast)
    • Ubiquitin conjugating enzyme UbcE2A
    • Ubiquitin like protein SUMO 1 conjugating enzyme
    • Ubiquitin protein ligase E2I
    • Ubiquitin-conjugating enzyme E2 I
    • Ubiquitin-protein ligase I
    see all

  • Function

    Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation.

  • Tissue specificity

    Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.

  • Pathway

    Protein modification; protein sumoylation.

  • Sequence similarities

    Belongs to the ubiquitin-conjugating enzyme family.

  • Cellular localization

    Nucleus. Cytoplasm. Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body.
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab173073? Please let us know so that we can cite the reference in this datasheet.

ab173073 has not yet been referenced specifically in any publications.

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