Recombinant Human UBE2I / UBC9 protein (ab151910)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% Densitometry
- Tags: His tag N-Terminus
- Suitable for: WB, SDS-PAGE
Description
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Product name
Recombinant Human UBE2I / UBC9 protein
See all UBE2I / UBC9 proteins and peptides -
Purity
> 95 % Densitometry. -
Expression system
Escherichia coli -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKG TPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEED KDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRA QAKKFAPS -
Predicted molecular weight
19 kDa including tags -
Amino acids
1 to 158 -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab151910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.00
Preservative: 1.02% Imidazole
Constituents: 0.002% PMSF, 0.82% Sodium phosphate, 0.04% DTT, 25% Glycerol, 1.75% Sodium chloride
General Info
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Alternative names
- C358B7.1
- p18
- SUMO 1 protein ligase
see all -
Function
Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. -
Tissue specificity
Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus. -
Pathway
Protein modification; protein sumoylation. -
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family. -
Cellular localization
Nucleus. Cytoplasm. Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab151910 has not yet been referenced specifically in any publications.