Recombinant Human UBE2I / UBC9 protein (ab151910)

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SDS-PAGE - Recombinant Human UBE2I / UBC9 protein (ab151910)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% Densitometry
    • Tags: His tag N-Terminus
    • Suitable for: WB, SDS-PAGE

    Description

    • Product name

      Recombinant Human UBE2I / UBC9 protein
      See all UBE2I / UBC9 proteins and peptides

    • Purity

      > 95 % Densitometry.

    • Expression system

      Escherichia coli

    • Accession

      P63279

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKG TPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEED KDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRA QAKKFAPS

      • Predicted molecular weight

        19 kDa including tags

      • Amino acids

        1 to 158

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab151910 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Western blot

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 7.00
      Preservative: 1.02% Imidazole
      Constituents: 0.002% PMSF, 0.82% Sodium phosphate, 0.04% DTT, 25% Glycerol, 1.75% Sodium chloride

    General Info

    • Alternative names

      • C358B7.1
      • p18
      • SUMO 1 protein ligase
      • SUMO conjugating enzyme UBC9
      • SUMO-conjugating enzyme UBC9
      • SUMO-protein ligase
      • SUMO1 protein ligase
      • UBC9
      • UBC9_HUMAN
      • UBCE9
      • Ube2i
      • Ubiquitin carrier protein
      • Ubiquitin carrier protein 9
      • Ubiquitin carrier protein I
      • Ubiquitin conjugating enzyme 9
      • Ubiquitin conjugating enzyme E2I (homologous to yeast UBC9)
      • Ubiquitin conjugating enzyme E2I (UBC9 homolog, yeast)
      • Ubiquitin conjugating enzyme UbcE2A
      • Ubiquitin like protein SUMO 1 conjugating enzyme
      • Ubiquitin protein ligase E2I
      • Ubiquitin-conjugating enzyme E2 I
      • Ubiquitin-protein ligase I
      see all

    • Function

      Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation.

    • Tissue specificity

      Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.

    • Pathway

      Protein modification; protein sumoylation.

    • Sequence similarities

      Belongs to the ubiquitin-conjugating enzyme family.

    • Cellular localization

      Nucleus. Cytoplasm. Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human UBE2I / UBC9 protein (ab151910)
      SDS-PAGE - Recombinant Human UBE2I / UBC9 protein (ab151910)
      SDS-PAGE analysis of ab151910.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab151910? Please let us know so that we can cite the reference in this datasheet.

    ab151910 has not yet been referenced specifically in any publications.

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