Recombinant Human UBE2I / UBC9 protein (ab151910)

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Description

  • Product name

    Recombinant Human UBE2I / UBC9 protein
    See all UBE2I / UBC9 proteins and peptides

  • Purity

    > 95 % Densitometry.

  • Expression system

    Escherichia coli

  • Accession

    P63279

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKG TPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEED KDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRA QAKKFAPS

    • Predicted molecular weight

      19 kDa including tags

    • Amino acids

      1 to 158

    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab151910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.00
    Preservative: 1.02% Imidazole
    Constituents: 0.002% PMSF, 0.82% Sodium phosphate, 0.04% DTT, 25% Glycerol, 1.75% Sodium chloride

General Info

  • Alternative names

    • C358B7.1
    • p18
    • SUMO 1 protein ligase
    • SUMO conjugating enzyme UBC9
    • SUMO-conjugating enzyme UBC9
    • SUMO-protein ligase
    • SUMO1 protein ligase
    • UBC9
    • UBC9_HUMAN
    • UBCE9
    • Ube2i
    • Ubiquitin carrier protein
    • Ubiquitin carrier protein 9
    • Ubiquitin carrier protein I
    • Ubiquitin conjugating enzyme 9
    • Ubiquitin conjugating enzyme E2I (homologous to yeast UBC9)
    • Ubiquitin conjugating enzyme E2I (UBC9 homolog, yeast)
    • Ubiquitin conjugating enzyme UbcE2A
    • Ubiquitin like protein SUMO 1 conjugating enzyme
    • Ubiquitin protein ligase E2I
    • Ubiquitin-conjugating enzyme E2 I
    • Ubiquitin-protein ligase I
    see all

  • Function

    Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation.

  • Tissue specificity

    Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.

  • Pathway

    Protein modification; protein sumoylation.

  • Sequence similarities

    Belongs to the ubiquitin-conjugating enzyme family.

  • Cellular localization

    Nucleus. Cytoplasm. Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human UBE2I / UBC9 protein (ab151910)
    SDS-PAGE - Recombinant Human UBE2I / UBC9 protein (ab151910)
    SDS-PAGE analysis of ab151910.

References

ab151910 has not yet been referenced specifically in any publications.

Publishing research using ab151910? Please let us know so that we can cite the reference in this datasheet.

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