Recombinant Human UBE2M/UBC12 protein (ab185866)

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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Endotoxin level: < 1.000 Eu/µg
  • Suitable for: HPLC, SDS-PAGE

Description

  • Product name

    Recombinant Human UBE2M/UBC12 protein
    See all UBE2M/UBC12 proteins and peptides

  • Purity

    > 95 % SDS-PAGE.
    Purity is greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    Escherichia coli

  • Accession

    P61081

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCDIS FSDPDDLLNFKLVICPDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVY HPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAA EVLQNNRRLFEQNVQRSMRGGYIGSTYFERCLK

    • Predicted molecular weight

      21 kDa

    • Amino acids

      1 to 183

Associated products

Specifications

Our Abpromise guarantee covers the use of ab185866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    HPLC

    SDS-PAGE

  • Form

    Liquid

  • Additional notes

     This product was previously labelled as UBE2M

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 1.19% HEPES, 0.03% DTT, 0.88% Sodium chloride, 10% Glycerol (glycerin, glycerine)

General Info

  • Alternative names

    • hUbc12
    • NEDD8 carrier protein
    • NEDD8 conjugating enzyme Ubc12
    • NEDD8 protein ligase
    • NEDD8-conjugating enzyme Ubc12
    • UBC-RS2
    • UBC12
    • UBC12_HUMAN
    • ube2m
    • ubiquitin carrier protein M
    • Ubiquitin conjugating enzyme E2 M
    • Ubiquitin-conjugating enzyme E2 M
    • ubiquitin-protein ligase M
    • yeast UBC12 homolog
    see all

  • Function

    Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.

  • Pathway

    Protein modification; protein neddylation.

  • Sequence similarities

    Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.

  • Domain

    Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8.
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab185866? Please let us know so that we can cite the reference in this datasheet.

ab185866 has not yet been referenced specifically in any publications.

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