Recombinant Human UBE2O protein (BSA and azide free) (ab172183)

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SDS-PAGE - Recombinant Human UBE2O protein (BSA and azide free) (ab172183)

    Key features and details

    • Expression system: Baculovirus infected Sf9 cells
    • Purity: > 95% Densitometry
    • Tags: proprietary tag N-Terminus
    • Suitable for: SDS-PAGE, WB

    Description

    • Product name

      Recombinant Human UBE2O protein (BSA and azide free)
      See all UBE2O proteins and peptides

    • Purity

      > 95 % Densitometry.

    • Expression system

      Baculovirus infected Sf9 cells

    • Accession

      BC022237

    • Protein length

      Full length protein

    • Animal free

      No

    • Carrier free

      Yes

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIVEE EKMEAVPDVERKEDKPEGQSPVKAEWPSET PVLCQQCGGKPGVTFTSA KGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPEGIM VKTFEDRMDLFS ALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYL SQCSGRLNPNLYDNGKVCVSLLGTCIGKGTERWTSKSSLLQVLI SIQG LILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPP EVFEQEIRQHFSTGGWRLVNRIESWL ETHALLEKAQALPNGVPKASSS PEPPAVAELSDSGQQEPEDGGPAPGEASQGSDSEGGAQSLASASRDHTDQ TSETAPDA SVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFI KSIRGVLTQFRAALLEAGMPECTEDK

      • Predicted molecular weight

        51 kDa including tags

      • Amino acids

        1 to 466

      • Tags

        proprietary tag N-Terminus

    Associated products

    Specifications

    Our Abpromise guarantee covers the use of ab172183 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Western blot

    • Form

      Liquid

    • Additional notes

      Previously labelled as E2 230K. 

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

      pH: 7.50
      Constituents: 0.79% Tris-HCl buffer, 25% Glycerol (glycerin, glycerine), 0.29% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF

    General Info

    • Alternative names

      • E2 230K
      • EC=6.3.2.19
      • FLJ12878
      • KIAA1734
      • Likely Ortholog of Mouse Ubiquitin Conjugating Enzyme
      • RP23 193A16.5
      • UBE2O
      • UBE2O_HUMAN
      • Ubiquitin carrier protein O
      • Ubiquitin conjugating enzyme E2 O
      • Ubiquitin conjugating enzyme E2 of 230 kDa
      • ubiquitin conjugating enzyme E2O
      • Ubiquitin protein ligase O
      • Ubiquitin-conjugating enzyme E2 O
      • Ubiquitin-conjugating enzyme E2 of 230 kDa
      • Ubiquitin-conjugating enzyme E2-230K
      • Ubiquitin-protein ligase O
      see all

    • Function

      Catalyzes the covalent attachment of ubiquitin to other proteins.

    • Tissue specificity

      Predominantly expressed in skeletal muscle and heart.

    • Pathway

      Protein modification; protein ubiquitination.

    • Sequence similarities

      Belongs to the ubiquitin-conjugating enzyme family.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human UBE2O protein (BSA and azide free) (ab172183)
      SDS-PAGE - Recombinant Human UBE2O protein (BSA and azide free) (ab172183)

      ab172183 on SDS-PAGE, MW ~90 kDa.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab172183? Please let us know so that we can cite the reference in this datasheet.

    ab172183 has not yet been referenced specifically in any publications.

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