Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 81% SDS-PAGE
- Active: Yes
- Tags: His-DDDDK tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human UBE3A protein
See all UBE3A proteins and peptides
Assay Conditions: 50 µl of reaction mixture 4 mM ATP, 5 mM MgCl2, 1 mM DTT, E1 (0.5 µg), E2 (2.8 µg), Biotinylated Ubiquitin (1 µg) in 50 mM Tris pH 7.4 was incubated for 30 min at 37°C. Transfer the reaction mixture to a streptavidin-coated plate and incubate for 30 min at 37°C. Add antibody against UBE3A, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence.
Purity>= 81 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
SequenceKRAAAKHLIERYYHQLTEGCGNEACTNEFCASCPTFLRMDNNAAAIKALE LYKINAKLCDPHPSKKGASSAYLENSKGAPNNSCSEIKMNKKGARIDFKD VTYLTEEKVYEILELCREREDYSPLIRVIGRVFSSAEALVQSFRKVKQHT KEELKSLQAKDEDKDEDEKEKAACSAAAMEEDSEASSSRIGDSSQGDNNL QKLGPDDVSVDIDAIRRVYTRLLSNEKIETAFLNALVYLSPNVECDLTYH NVYSRDPNYLNLFIIVMENRNLHSPEYLEMALPLFCKAMSKLPLAAQGKL IRLWSKYNADQIRRMMETFQQLITYKVISNEFNSRNLVNDDDAIVAASKC LKMVYYANVVGGEVDTNHNEEDDEEPIPESSELTLQELLGEERRNKKGPR VDPLETELGVKTLDCRKPLIPFEEFINEPLNEVLEMDKDYTFFKVETENK FSFMTCPFILNAVTKNLGLYYDNRIRMYSERRITVLYSLVQGQQLNPYLR LKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVSKEF FQLVVEEIFNPDIGMFTYDESTKLFWFNPSSFETEGQFTLIGIVLGLAIY NNCILDVHFPMVVYRKLMGKKGTFRDLGDSHPVLYQSLKDLLEYEGNVED DMMITFQISQTDLFGNPMMYDLKENGDKIPITNENRKEFVNLYSDYILNK SVEKQFKAFRRGFHMVTNESPLKYLFRPEEIELLICGSRNLDFQALEETT EYDGGYTRDSVLIREFWEIVHSFTDEQKRLFLQFTTGTDRAPVGGLGKLK MIIAKNGPDTERLPTSHTCFNVLLLPEYSSKEKLKERLLKAITYAKGFGM L
Predicted molecular weight100 kDa including tags
Amino acids2 to 852
TagsHis-DDDDK tag N-Terminus
Additional sequence informationIsoform I. NM_130838.
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1257)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab21536)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-6X His tag® antibody (ab9108)
Our Abpromise guarantee covers the use of ab198417 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol, 0.05% DTT
Contains 80 µg/ml DDDDK peptide.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Angelman syndrome
FunctionE3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Catalyzes the high-risk human papilloma virus E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo. Plays an important role in the regulation of the circadian clock: involved in the ubiquitination of the core clock component ARNTL/BMAL1, leading to its proteasomal degradation (PubMed:24728990).
PathwayProtein modification; protein ubiquitination.
Involvement in diseaseAngelman syndrome
Sequence similaritiesContains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
modificationsPhosphorylation at Tyr-659 by ABL1 impairs E3 ligase activity and protects p53/TP53 from degradation in (HPV)-infected cells.
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab198417 has not yet been referenced specifically in any publications.