Recombinant human Ubiquitin 7-amido-4-methylcoumarin protein (ab206162)

Description

  • Product name

    Recombinant human Ubiquitin 7-amido-4-methylcoumarin protein
  • Biological activity

    The concentration of ab206162 used in deubiquitinating reactions is usually 0.1-1 µM. The released AMC fluorescence can be monitored using a fluorometer or plate reader with excitation and emission wavelengths at 360 nm and 460 nm, respectively.

  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQL EDGRTLSDYN IQKESTLHLVLRLRGG
    • Predicted molecular weight

      9 kDa
    • Amino acids

      1 to 76

Specifications

Our Abpromise guarantee covers the use of ab206162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    Avoid mixing with compound or buffer containing free amino group.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Ub-AMC
    • Ubiquitin-AMC
  • Relevance

    Ubiquitin 7-amido-4-methylcoumarin (Ub-AMC) is a sensitive fluorescence-based substrate of deubiquitinating enzymes. It is useful for monitoring deubiquitinating enzymes’ activity or screening inhibitors of deubiquitinating enzymes. The concentration of Ub-AMC used in deubiquitinating reactions is usually 0.1-1 µM. The released AMC fluorescence can be monitored using a fluorometer or plate reader with excitation and emission wavelengths at 360 nm and 460 nm, respectively.
  • Cellular localization

    Cytoplasmic and Nuclear

Images

  • Functional analysis of ab206162: The reaction contained 2 nM bovine 26S proteasome and 1 mM ab206162. AMC fluorescence was monitored by a plate reader using excitation and emission filter set at 360/40 and 460/30 nm, respectively.

  • SDS-PAGE analysis of ab206162, Coomassie-stained (5 µg).

References

ab206162 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab206162.
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