Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, MS, Functional Studies, SDS-PAGE
Product nameRecombinant human Ubiquitin K33 only protein
Purity> 95 % SDS-PAGE.
High molecular weight conjugates were separated from free ubiquitin by size exclusion chromatography.
Expression systemEscherichia coli
Protein lengthFull length protein
Our Abpromise guarantee covers the use of ab157076 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.02% DTT, 0.61% Tris, 0.88% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Information by UniProt
Coomassie stained SDS-PAGE gel showing ubiquitinylation of substrate protein (X) with (K33 only) Ub to give (K33 only)Ubn-ubiquitinylated conjugates. Z ab157056.
polyubiquitin-chain reactive monoclonal antibody FK2 at 1/1000 dilution +
Recombinant human Ubiquitin K33 only protein (ab157076)
All lanes : PAb to ubiquitin-protein conjugates at 1/1000 dilution
Lane 1 : ab157076 incubated with USP2cd
Lane 2 : ab157076 incubated without USP2cd
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab157076 has not yet been referenced specifically in any publications.