Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE, MS
Product nameRecombinant human Ubiquitin K48 only protein
Biological activityDUB reactivity: The potential utility of (K48-only) Ubn-ubiquitinylated conjugates as DUB substrates was demonstrated by their deconjugation in the presence of the isolated catalytic domain of USP2, a DUB of general and promiscuous activity that appears to exhibit no linkage preference.
Purity> 95 % SDS-PAGE.
High molecular weight conjugates were separated from free ubiquitin by size exclusion chromatography.
Expression systemEscherichia coli
Protein lengthFull length protein
Our Abpromise guarantee covers the use of ab157048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.61% Tris, 0.88% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
FunctionUbiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
Sequence similaritiesBelongs to the ubiquitin family.
Contains 3 ubiquitin-like domains.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
Coomassie stained SDS-PAGE gel showing ubiquitinylation of substrate protein (X) with (K48 only) Ub to give (K48 only) Ubn-ubiquitinylated conjugates (Z).
monoclonal antibody FK2 at 1/1000 dilution +
Recombinant human Ubiquitin K48 only protein (ab157048)
All lanes : PAb to ubiquitin-protein conjugates at 1/1000 dilution
Lane 1 : ab157048 incubated with USP2cd
Lane 2 : ab157048 incubated without USP2cd
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab157048 has not yet been referenced specifically in any publications.