The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Ub-AML gives a strong luminescent signal in the range of 0.1-1 μM, depending on exact experimental conditions. Optimal luminescence at pH 7.5 is monitored using all wavelengths with a 500 ms integration time.
Activity: Over 100:1 signal to noise ratio with 10 pM UCH-L3.
>95% by SDS-PAGE .
Substrate for deubiquitinating enzymes based on the C-terminal derivative of ubiquitin with aminoluciferin (AML). Rather than fluorescence as the indicator of DUB activity, DUB liberated luciferin is processed by luciferase to give a luminescence signal. Ub-AML not only produces a stronger signal, but also has an excellent signal to noise ratio over traditional fluorophores.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.5 Constituent: 1.19% HEPES
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Epididymis secretory protein Li 50
HEL S 50
Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
Similarity: Belongs to the ubiquitin family.
Contains 3 ubiquitin-like domains.
Cell Membrane, Cytoplasmic and Nuclear
has not yet been referenced specifically in any publications.
Publishing research using ab189229? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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