Recombinant Human UBPY/USP8 protein (ab127607)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His-DHFR tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human UBPY/USP8 protein
See all UBPY/USP8 proteins and peptides -
Purity
> 95 % SDS-PAGE.
Purified via His tag -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
25 kDa -
Amino acids
886 to 1096 -
Tags
His-DHFR tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab127607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20ºC.
Constituents: 0.32% Tris HCl, 0.58% Sodium chloride
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ReconstitutionReconstitute with water to desired concentration.
General Info
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Alternative names
- Deubiquitinating enzyme 8
- FLJ34456
- hUBPy
see all -
Function
Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1. -
Sequence similarities
Belongs to the peptidase C19 family.
Contains 1 MIT domain.
Contains 1 rhodanese domain.
Contains 1 USP domain. -
Domain
The MIT domain is required for endosomal localization, CHMP1B-binding, maintenance of ESCRT-0 stability and EGFR degradation.
The rhodanese domain is sufficient for RNF41-binding. -
Post-translational
modificationsPhosphorylation of Ser-718 is essential for interaction with YWHAE and for cytosol localization. Undergoes dephosphorylation at Ser-718 in the M phase. Tyrosine-phosphorylated in its N-terminal half in an EGFR-dependent manner.
Ubiquitinated. Inactive form is mostly monoubiquitinated, but polyubiquitination happens too. Ubiquitination is increased in EGF-stimulated cells. Ubiquitination of active form is undetectable, suggesting a possibility that USP8 deubiquitinates itself, thereby regulating its own function. -
Cellular localization
Cytoplasm. Nucleus. Endosome membrane. Cell membrane. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab127607 has been referenced in 1 publication.
- Mao G et al. Strontium-substituted Nanohydroxyapatite Coatings to Decrease Aseptic Loosening of Femoral Prosthesis: Safety and Efficacy in a Rat Model. ACS Appl Mater Interfaces N/A:N/A (2019). PubMed: 30763063