The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Specific activity: >3,000 pmole/min/ug. Measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37°C. Activity Assay:
Prepare a 100ul of recombinant UCH-L3 protein with various concentrations (0.48ng, 0.9ng) in assay buffer and equilibrate to 37°C for 10 minutes. (Assay buffer: 50mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 0.1 mg/ml Ovalbumin, pH 8.0.)
Add 50ul of 1uM Ubiquitin-AMC.
Read at excitation wavelengths 355nm and emission 460nm for 5 minutes.
- 96 Well Polystyrene Microplate, black
- Fluorescent plate reader (PerkinElmer, VICTOR X3)
% SDS-PAGE. ab103502 purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 8.00 Constituents: 0.0154% DTT, 0.316% Tris HCl, 10% Glycerol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Ubiquitin carboxyl-terminal hydrolase isozyme L3
Ubiquitin thioesterase L3
Ubiquitin thiolesterase L3
Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3". Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory.
Highly expressed in heart, skeletal muscle, and testis.
Belongs to the peptidase C12 family.
Phosphorylated upon DNA damage, probably by ATM or ATR.