Description

  • Product name

    Recombinant Human ULK1 protein
    See all ULK1 proteins and peptides
  • Expression system

    HEK 293 cells
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      113 kDa including tags
    • Amino acids

      2 to 1050
    • Tags

      DDDDK tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab95322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 0.0462% DTT, 0.395% Tris HCl, 0.05% Tween, 20% Glycerol, 0.58% Sodium chloride

General Info

  • Alternative names

    • ATG 1
    • ATG1
    • ATG1 autophagy related 1 homolog
    • ATG1A
    • Autophagy related protein 1 homolog
    • Autophagy-related protein 1 homolog
    • FLJ38455
    • FLJ46475
    • hATG1
    • KIAA0722
    • Serine/threonine protein kinase ULK1
    • Serine/threonine protein kinase Unc51.1
    • Serine/threonine-protein kinase ULK1
    • ULK 1
    • ULK1
    • ULK1_HUMAN
    • UNC 51
    • Unc 51 (C. elegans) like kinase 1
    • Unc 51 like kinase 1
    • Unc-51 like kinase 1 (C. elegans)
    • Unc-51-like kinase 1
    • UNC51
    • UNC51, C. elegans, homolog of
    • Unc51.1
    see all
  • Function

    Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165).
  • Tissue specificity

    Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated. Phosphorylated under nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency, phosphorylated by MTOR/mTOR, disrupting the interaction with AMPK and preventing activation of ULK1 (By similarity). In response to nutrient limitation, phosphorylated and activated by AMPK, leading to activate autophagy.
  • Cellular localization

    Cytoplasm, cytosol. Preautophagosomal structure. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.
  • Information by UniProt

Images

  • 10% SDS-PAGE analysis
    Lane 1: 3 µg ab95322
    Lane 2: Molecular Weight Markers

References

ab95322 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Thank you for contacting Abcam.

Attached please find a WB image confirming the protein.

I hope this information is helpful.

Please do not hesitate to contact us if you have any additional questions.

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Answer

Thank you for contacting Abcam regarding ab95322.

I have confirmed that yes, we have the western blot data to confirm this protein band. Iam working on getting an image from the lab for you. We don’t know the identity of the other bands, but it is likely contaminating proteins.

I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

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