Recombinant human uPA protein (ab167714)
- Datasheet
- References
- Protocols
Overview
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Product nameRecombinant human uPA protein
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Protein lengthFull length protein
Description
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NatureRecombinant
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SourceHEK 293 cells
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Amino Acid Sequence
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Accession
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SpeciesHuman
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SequenceSNELHQVPSNCDCLNGGTCVSNKYFSNIHWCNCPKKFGGQHCEIDKSKTC YEGNGHFYRGKASTDTMGRPCLPWNSATVLQQTYHAHRSDALQLGLGKHN YCRNPDNRRRPWCYVQVGLKPLVQECMVHDCADGKKPSSPPEELKFQCGQ KTLRPRFKIIGGEFTTIENQPWFAAIYRRHRGGSVTYVCGGSLISPCWVI SATHCFIDYPKKEDYIVYLGRSRLNSNTQGEMKFEVENLILHKDYSADTL AHHNDIALLKIRSKEGRCAQPSRTIQTICLPSMYNDPQFGTSCEITGFGK ENSTDYLYPEQLKMTVVKLISHRECQQPHYYGSEVTTKMLCAADPQWK TDSCQGDSGGPLVCSLQGRMTLTGIVSWGRGCALKDKPGVYTRVSHFLPW IRSHTKEENGLAL
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Molecular weight45 kDa including tags
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Amino acids21 to 431
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TagsHis tag C-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab167714 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Biological activityMeasured by its ability to cleave a peptide substrate, N - carbobenzyloxy - Gly - Gly - Arg - 7 - amido - 4 - methylcoumarin (Z - GGR - AMC). The specific activity is > 2000 pmoles/min/µg.
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Applications
SDS-PAGE
Functional Studies
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Endotoxin level< 1.000 Eu/µg
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Purity>95% by SDS-PAGE .
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FormLyophilised
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituent: 99% PBS
Note: Normally Mannitol or Trehalose are added as protectants before lyophilization.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionIt is recommended to reconstitute the lyophilized protein in 50µl sterile deionized water to a final concentration of 1mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
General Info
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Alternative names
- ATF
- BDPLT5
- EC 3.4.21.73
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RelevanceUrokinase type plasminogen activator (uPA) is secreted from cells as a precursor which is activated to the two chain form consisting of an A and B chain. The active high MW form is further processed by removal of an amino terminal fragment to an active low MW form (35 kDa). uPA is a serine protease that activates plasminogen toplasmin. High levels of uPA and plasminogen activator inhibitor type 1 (PAI 1) in breast cancer tissue extracts have been associated with rapid disease progression. The malignant phenotype of prostatic tumor cells correlates with the expression of both uPA and its cell membrane receptor (uPAR).
Images
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SDS-PAGE - Recombinant human uPA protein (ab167714)SDS-PAGE of reduced ab167714 stained overnight with Coomassie Blue.
DTT-reduced Protein migrates as three bands corresponding to the long α chain, β chain and unprocessed full-length chain with the molecular mass of 18 kDa, 32 kDa and 50 kDa respectively due to glycosylation and cleavage.
Datasheets and documents
References
ab167714 has not yet been referenced specifically in any publications.
