Recombinant Human USP1 protein (ab132351)
Key features and details
- Expression system: Wheat germ
- Suitable for: WB, SDS-PAGE, ELISA
Description
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Product name
Recombinant Human USP1 protein -
Expression system
Wheat germ -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MPGVIPSESNGLSRGSPSKKNRLSLKFFQKKETKRALDFTDSQENEEKAS EYRASEIDQVVPAAQSSPINCEKRENLLPFVGLNNLGNTCYLNSILQVLY FCPGFKSGVKHLFNIISRKKEALKDEANQKDKGNCKEDSLASYELICSLQ SLIISVEQLQASFLLNPEKYTDELATQPRRLLNTLRELNPMYEGYLQHDA QEVLQCILGNIQETCQLLKKEEVKNVAELPTKVEEIPHPKEEMNGINSIE MDSMRHSEDFKEKLPKGNGKRKSDTEFGNMKKKVKLSKEHQSLEENQRQT RSKRKATSDTLESPPKIIPKYISENESPRPSQKKSRVKINWLKSATKQPS ILSKFCSLGKITTNQGVKGQSKENECDPEEDLGKCESDNTTNGCGLESPG NTVTPVNVNEVKPINKGEEQIGFELVEKLFQGQLVLRTRCLECESLTERR EDFQDISVPVQEDELSKVEESSEISPEPKTEMKTLRWAISQFASVERIVG EDKYFCENCHHYTEAERSLLFDKMPEVITIHLKCFAASGLEFDCYGGGLS KINTPLLTPLKLSLEEWSTKPTNDSYGLFAVVMHSGITISSGHYTASVKV TDLNSLELDKGNFVVDQMCEIGKPEPLNEEEARGVVENYNDEEVSIRVGG NTQPSKVLNKKNVEAIGLLGGQKSKADYELYNKASNPDKVASTAFAENRN SETSDTTGTHESDRNKESSDQTGINISGFENKISYVVQSLKEYEGKWLLF DDSEVKVTEEKDFLNSLSPSTSPTSTPYLLFYKKL -
Predicted molecular weight
115 kDa including tags -
Amino acids
1 to 785
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab132351 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
SDS-PAGE
ELISA
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HCl
General Info
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Alternative names
- Deubiquitinating enzyme 1
- hUBP
- Ubiquitin carboxyl terminal hydrolase 1
see all -
Function
Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. -
Sequence similarities
Belongs to the peptidase C19 family. -
Developmental stage
Cell cycle-regulated. Highest level during S phase. -
Post-translational
modificationsAutocatalytic cleavage of USP1 following UV irradiation inactivates it leading to an increase in ubiquitinated PCNA, recruitment of POLH and translesion synthesis.
Ubiquitinated; leading to its subsequent proteasomal degradation.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab132351 has not yet been referenced specifically in any publications.