Recombinant Human USP1 protein (ab132351)

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Description

  • Product name

    Recombinant Human USP1 protein

  • Expression system

    Wheat germ

  • Accession

    O94782

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MPGVIPSESNGLSRGSPSKKNRLSLKFFQKKETKRALDFTDSQENEEKAS EYRASEIDQVVPAAQSSPINCEKRENLLPFVGLNNLGNTCYLNSILQVLY FCPGFKSGVKHLFNIISRKKEALKDEANQKDKGNCKEDSLASYELICSLQ SLIISVEQLQASFLLNPEKYTDELATQPRRLLNTLRELNPMYEGYLQHDA QEVLQCILGNIQETCQLLKKEEVKNVAELPTKVEEIPHPKEEMNGINSIE MDSMRHSEDFKEKLPKGNGKRKSDTEFGNMKKKVKLSKEHQSLEENQRQT RSKRKATSDTLESPPKIIPKYISENESPRPSQKKSRVKINWLKSATKQPS ILSKFCSLGKITTNQGVKGQSKENECDPEEDLGKCESDNTTNGCGLESPG NTVTPVNVNEVKPINKGEEQIGFELVEKLFQGQLVLRTRCLECESLTERR EDFQDISVPVQEDELSKVEESSEISPEPKTEMKTLRWAISQFASVERIVG EDKYFCENCHHYTEAERSLLFDKMPEVITIHLKCFAASGLEFDCYGGGLS KINTPLLTPLKLSLEEWSTKPTNDSYGLFAVVMHSGITISSGHYTASVKV TDLNSLELDKGNFVVDQMCEIGKPEPLNEEEARGVVENYNDEEVSIRVGG NTQPSKVLNKKNVEAIGLLGGQKSKADYELYNKASNPDKVASTAFAENRN SETSDTTGTHESDRNKESSDQTGINISGFENKISYVVQSLKEYEGKWLLF DDSEVKVTEEKDFLNSLSPSTSPTSTPYLLFYKKL

    • Predicted molecular weight

      115 kDa including tags

    • Amino acids

      1 to 785

Specifications

Our Abpromise guarantee covers the use of ab132351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

    ELISA

  • Form

    Liquid

  • Additional notes

    This protein is best used within three months from the date of receipt.

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names

    • Deubiquitinating enzyme 1
    • hUBP
    • Ubiquitin carboxyl terminal hydrolase 1
    • Ubiquitin carboxyl-terminal hydrolase 1
    • Ubiquitin specific peptidase 1
    • Ubiquitin specific protease 1
    • Ubiquitin thioesterase 1
    • Ubiquitin thiolesterase 1
    • Ubiquitin-specific-processing protease 1
    • UBP
    • UBP1_HUMAN
    • USP 1
    • Usp1
    see all

  • Function

    Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity.

  • Sequence similarities

    Belongs to the peptidase C19 family.

  • Developmental stage

    Cell cycle-regulated. Highest level during S phase.

  • Post-translational
    modifications

    Autocatalytic cleavage of USP1 following UV irradiation inactivates it leading to an increase in ubiquitinated PCNA, recruitment of POLH and translesion synthesis.
    Ubiquitinated; leading to its subsequent proteasomal degradation.
    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Nucleus.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human USP1 protein (ab132351)
    SDS-PAGE - Recombinant Human USP1 protein (ab132351)
    12.5% SDS-PAGE analysis of ab132351 stained with Coomassie Blue.

References

ab132351 has not yet been referenced specifically in any publications.

Publishing research using ab132351? Please let us know so that we can cite the reference in this datasheet.

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