Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 60% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human USP10 protein
Specific Activity: 23 pmol/min/µg.
Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and ab198669 in a 50 µL volume. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, emission = 460 nm.
Purity>= 60 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
SequenceALHSPQYIFGDFSPDEFNQFFVTPRSSVELPPYSGTVLCGTQAVDKLPDG QEYQRIEFGVDEVIEPSDTLPRTPSYSISSTLNPQAPEFILGCTASKITP DGITKEASYGSIDCQYPGSALALDGSSNVEAEVLENDGVSGGLGQRERKK KKKRPPGYYSYLKDGGDDSISTEALVNGHANSAVPNSVSAEDAEFMGDMP PSVTPRTCNSPQNSTDSVSDIVPDSPFPGALGSDTRTAGQPEGGPGADFG QSCFPAEAGRDTLSRTAGAQPCVGTDTTENLGVANGQILESSGEGTATNG VELHTTESIDLDPTKPESASPPADGTGSASGTLPVSQPKSWASLFHDSKP SSSSPVAYVETKYSPPAISPLVSEKQVEVKEGLVPVSEDPVAIKIAELLE NVTLIHKPVSLQPRGLINKGNWCYINATLQALVACPPMYHLMKFIPLYSK VQRPCTSTPMIDSFVRLMNEFTNMPVPPKPRQALGDKIVRDIRPGAAFEP TYIYRLLTVNKSSLSEKGRQEDAEEYLGFILNGLHEEMLNLKKLLSPSNE KLTISNGPKNHSVNEEEQEEQGEGSEDEWEQVGPRNKTSVTRQADFVQTP ITGIFGGHIRSVVYQQSSKESATLQPFFTLQLDIQSDKIRTVQDALESLV ARESVQGYTTKTKQEVEISRRVTLEKLPPVLVLHLKRFVYEKTGGCQKLI KNIEYPVDLEISKELLSPGVKNKNFKCHRTYRLFAVVYHHGNSATGGHYT TDVFQIGLNGWLRIDDQTVKVINQYQVVKPTAERTAYLLYYRRVDLL
Predicted molecular weight88 kDa including tags
Amino acids2 to 798
TagsDDDDK tag N-Terminus
Our Abpromise guarantee covers the use of ab198669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.71% Tris HCl, 0.72% Sodium chloride, 0.02% Potassium chloride, 10% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Contains 90 µg/mL DDDDK peptide
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Deubiquitinating enzyme 10
FunctionHydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the peptidase C19 family. USP10 subfamily.
modificationsPhosphorylated by ATM following DNA damage, leading to stablization and translocation it to the nucleus.
Cellular localizationCytoplasm. Nucleus. Early endosome. Cytoplasmic in normal conditions. After DNA damage, translocates to the nucleus following phosphorylation by ATM.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab198669 has not yet been referenced specifically in any publications.