Recombinant human USP14/TGT protein (ab198432)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 75% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human USP14/TGT protein
See all USP14/TGT proteins and peptides -
Biological activity
Specific Activity: 56 pmol/min/µg.
Assay Conditions: Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and USP14 in a 50 µL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, emission = 460 nm.
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Purity
>= 75 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
PLYSVTVKWGKEKFEGVELNTDEPPMVFKAQLFALTGVQPARQKVMVKGG TLKDDDWGNIKIKNGMTLLMMGSADALPEEPSAKTVFVEDMTEEQLASAM ELPCGLTNLGNTCYMNATVQCIRSVPELKDALKRYAGALRASGEMASAQY ITAALRDLFDSMDKTSSSIPPIILLQFLHMAFPQFAEKGEQGQYLQQDAN ECWIQMMRVLQQKLEAIEDDSVKETDSSSASAATPSKKKSLIDQFFGVEF ETTMKCTESEEEEVTKGKENQLQLSCFINQEVKYLFTGLKLRLQEEITKQ SPTLQRNALYIKSSKISRLPAYLTIQMVRFFYKEKESVNAKVLKDVKFPL MLDMYELCTPELQEKMVSFRSKFKDLEDKKVNQQPNTSDKKSSPQKEVKY EPFSFADDIGSNNCGYYDLQAVLTHQGRSSSSGHYVSWVKRKQDEWIKFD DDKVSIVTPEDILRLSGGGDWHIAYVLLYGPRRVEIMEEESEQ -
Predicted molecular weight
57 kDa including tags -
Amino acids
2 to 494 -
Tags
DDDDK tag N-Terminus -
Additional sequence information
NM_005151.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab198432 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Additional notes
This product was previously labelled as USP14
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.71% Tris HCl, 0.72% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
90 µg/ml DDDDK peptideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Deubiquitinating enzyme 14
- TGT
- tRNA guanine transglycosylase
see all -
Function
Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs). -
Sequence similarities
Belongs to the peptidase C19 family. USP14/UBP6 subfamily.
Contains 1 ubiquitin-like domain. -
Cellular localization
Cytoplasm. Cell membrane. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab198432 has not yet been referenced specifically in any publications.